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Application of Rbm46 as marker of tumor of testis

A technology for testis and tumors, which is applied in the determination/testing of microorganisms, biomaterial analysis, biochemical equipment and methods, etc., and can solve the problems of lack of sensitivity and specificity, and inability to detect

Inactive Publication Date: 2019-09-03
BEIJING TAIDONG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the number of testicular germ cell tumors proliferates to 10 5 At that time, the existing imaging examinations could not detect it
At present, there is a lack of ideal tumor markers with sensitivity and specificity, so it is of great clinical significance to find testicular cancer markers with higher specificity

Method used

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  • Application of Rbm46 as marker of tumor of testis
  • Application of Rbm46 as marker of tumor of testis
  • Application of Rbm46 as marker of tumor of testis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Quantitative PCR was used to detect the expression of RBM46 gene in testicular cancer tissues.

[0059] Testicular cancer tissues and adjacent normal tissues were taken from the Cancer Hospital, placed in RNAlater reagent, and stored in a -80°C refrigerator. First, total cellular RNA was extracted. All manipulations were performed at low temperature.

[0060] Total RNA was extracted using Trizol reagent,

[0061] (1) Reagent preparation

[0062] 1. TRIzol reagent.

[0063] 2. Chloroform

[0064] 3. Isopropanol

[0065] 4. 75% ethanol (DEPC H 2 O preparation)

[0066] 5. DEPC H 2 o

[0067] Specific steps are as follows:

[0068] 1. Add 1ml Trizol to the tissue to fully homogenize it, and let it stand at room temperature for 5 minutes to fully lyse it.

[0069] 2. Add 0.2ml of chloroform, shake and mix well, and let it stand at room temperature for 10min.

[0070]3. Centrifuge at 4°C, 12000g×15min, and take the supernatant.

[0071] 4. Add 0.5ml of isopropan...

Embodiment 2

[0082] Detection of Rbm46 protein level (Western blot)

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Abstract

The invention relates to the field of cancer diagnosis and treatment, and in particular, relates to an application of Rbm46 as a marker of tumor of testis. The invention discloses an application of aquantitative detection agent of Rbm46 mRNA or proteins in preparation of reagents or kits for diagnosis and / or prognosis evaluation of tumor of testis, wherein reduced expression quantity of the Rbm46mRNA or proteins is an indication of tumor of testis and / or an indication of poor prognosis of tumor of testis. The measurement of the Rbm46 mRNA or proteins can be used for detecting or diagnosing tumor of testis, or for evaluating and monitoring the prognosis condition of patients, and has important clinical application value.

Description

technical field [0001] The present invention relates to the field of medical diagnosis, in particular, relates to the application of Rbm46 as a testicular tumor marker. Background technique [0002] Testicular tumor is one of the more common tumors of the urinary system. Most of the patients are young and middle-aged men, and almost all of them are malignant. Testicular cancer accounts for 1% of systemic malignancies and 3% to 9% of urogenital malignancies. The incidence of testicular cancer varies by race and region. The incidence of testicular cancer in whites is higher than that in non-whites. The incidence of testicular cancer in the United States is about 6 / 100,000, and the incidence in Africa is 1 / 100,000, and there is an increasing trend year by year. Testicular tumors are divided into two types: primary and secondary, most of which are primary, and secondary is extremely rare. Almost all testicular tumors are malignant, of which germ cell tumors account for 90% to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886G01N33/574
CPCC12Q1/6886C12Q2600/118C12Q2600/158G01N33/57407G01N33/57484G01N2800/52
Inventor 刘年
Owner BEIJING TAIDONG BIOTECH CO LTD