Preparation method and application of multi-metal-oxygen cluster-food-borne antioxidation peptide optothermal material
A polymetallic oxygen cluster, antioxidant peptide technology, applied in the direction of resisting vector-borne diseases, medical preparations containing active ingredients, wave energy or particle radiation treatment materials, etc., can solve the unstable state of heteropoly blue, reduce material Photothermal conversion efficiency, limited development and application, etc., achieve good physiological stability and biocompatibility, avoid inflammatory effects, and have broad application prospects.
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Embodiment 1
[0071] A preparation method of polymetallic oxygen cluster-food-derived antioxidant peptide photothermal material rSP, comprising the steps of:
[0072] S1. Soybean pentapeptide was synthesized by standard Fmoc solid-phase synthesis strategy, and its purity was measured by RP-C18 chromatographic column and matrix-assisted laser desorption / ionization time-of-flight mass spectrometry. The mass spectrometry results are as follows figure 1 Shown; Take the described soybean pentapeptide of 11.8mg and be dissolved in 10mL water, stir 10min, obtain the polypeptide aqueous solution that soybean pentapeptide concentration is 2mmol / L; The aminoacid sequence of described soybean pentapeptide is as shown in SEQ ID:1;
[0073] S2. Weigh 36.5 mg polymetallic oxygen cluster H 3 PMo 12 o 40 Dissolve in 10mL water and stir for 10min to obtain a polymetallic oxygen cluster aqueous solution with a concentration of 2mmol / L;
[0074] S3. Add the polymetallic oxygen cluster aqueous solution desc...
Embodiment 2
[0100] Cytotoxicity Test of Polymetallic Oxygen Clusters-Food Origin Antioxidant Peptide Photothermal Material rSP
[0101] HepG2 liver cancer tumor cells were selected for cytotoxicity test. HepG2 tumor cells were seeded in a 96-well culture plate, 10 per well 4 For each cell, select DMEM as the medium, inject 100uLDMEM solution into each well, and place in 5% CO 2 , and cultured in a cell culture incubator at 37°C for 24 hours. Take different concentrations of POM solution, rPOM solution and rSP solution samples (1250nM, 2500nM, 5000nM, 10000nM) 100uL were added to the cell wells, co-cultivated with HepG2 cells, and then continued to culture for 24 hours. Add 20uL MTT solution, cultivate for another 4 hours under the same conditions, suck out the culture solution in the wells, add 150uL DMSO to each well, and use a microplate reader to measure the absorption peak at 570nm. According to the OD of each experimental sample group value / OD value of the control group × 100%, ca...
Embodiment 3
[0103] In vitro treatment of HepG2 liver cancer tumor cells by polymetallic oxygen clusters-food-derived antioxidant peptide photothermal material rSP HepG2 liver cancer tumor cells were subjected to photothermal therapy. HepG2 tumor cells were seeded in a 96-well culture plate, and 10 cells were seeded in each well. 4cells, DMEM was selected as the medium, and placed in 5% CO 2 , and cultured in a cell culture incubator at 37°C for 24 hours. The rPOM solution, the rSP solution, the rPOM solution placed in the air for 24 hours, and the rSP solution placed in the air for 24 hours were added to the cell wells respectively, and co-cultivated with the HepG2 cells. After 1 hour, irradiate with an 808nm laser for 10 minutes, continue to cultivate for 24 hours, add MTT solution, add DMSO after cultivating for 4 hours under the same conditions, use a microplate reader to measure the absorption peak at the absorbance at 570nm, according to OD value = 1g (1 / trans), the cell survival r...
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