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A kind of injectable gelatin-sodium alginate porous cryogel with shape memory and preparation method thereof

A sodium alginate and cryogel technology, applied in the field of biomedical tissue engineering materials, can solve problems such as low mechanical strength and poor biocompatibility

Active Publication Date: 2021-11-09
CHONGQING UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] In order to achieve the purpose of the above invention, the present invention discloses an injectable gelatin-sodium alginate porous cryogel with shape memory and a preparation method thereof. The preparation method uses natural gelatin and sodium alginate as raw materials and adopts low-temperature freezing polymerization The preparation method obtains a new type of shape memory material, which can improve the problems of poor biocompatibility and low mechanical strength of existing cryogel materials

Method used

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  • A kind of injectable gelatin-sodium alginate porous cryogel with shape memory and preparation method thereof
  • A kind of injectable gelatin-sodium alginate porous cryogel with shape memory and preparation method thereof
  • A kind of injectable gelatin-sodium alginate porous cryogel with shape memory and preparation method thereof

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Embodiment 1

[0031] A preparation of an injectable gelatin-sodium alginate porous cryogel with shape memory, comprising the steps of:

[0032] 1) Weigh 70mg of methacrylic anhydride-modified gelatin and 5mg of sodium alginate dissolved in 1ml of PBS solution, put them in an oven at 25-60°C to dissolve them completely;

[0033] 2) Add 2.5ml of tetramethylethylenediamine to the solution obtained in step 1);

[0034] 3) Weigh 0.8g of ammonium persulfide (APS) and dissolve it in 8ml of PBS to form an APS solution with a concentration of 10% by mass;

[0035] 4) Add 50 μl of the APS solution obtained in step 3) to the mixture obtained in step 2), and mix it evenly;

[0036] 5) The mixture obtained in step 4) was sucked into a cylindrical silica gel mold with a diameter of 7.8 mm and a height of 5.3 mm in time, and placed in a freezer at -20° C. for 18 hours to prepare a gelatin-sodium alginate porous cryogel.

[0037] The hydrogel material that obtains by embodiment 1 is carried out electron ...

Embodiment 2

[0040] A preparation of an injectable gelatin-sodium alginate porous cryogel with shape memory, comprising the steps of:

[0041] 1) Weigh 70mg of methacrylic anhydride modified gelatin and 10mg of sodium alginate dissolved in 1ml of PBS solution, put them in an oven at 25-60°C to dissolve them completely;

[0042] 2) Add 2.5ml of tetramethylethylenediamine (TEMED) to the solution obtained in step 1);

[0043] 3) Weigh 0.8g of ammonium persulfide (APS) and dissolve it in 8ml of PBS to form an APS solution with a mass concentration of 10%;

[0044]4) Add 50 μl of the APS solution obtained in step 3) to the mixture obtained in step 2), and mix it evenly;

[0045] 5) The mixture obtained in step 4) was sucked into a cylindrical silica gel mold with a diameter of 7.8mm and a height of 5.3mm in time, and placed in a freezer at -20°C for 18 hours to prepare a gelatin-sodium alginate porous cryogel.

Embodiment 3

[0047] 1) Weigh 65mg of methacrylic anhydride-modified gelatin and 15mg of sodium alginate dissolved in 1ml of PBS solution, put them in an oven at 25-60°C to dissolve them completely;

[0048] 2) Add 2.5ml of tetramethylethylenediamine (TEMED) to the solution obtained in step 1);

[0049] 3) Weigh 0.8g of ammonium persulfide (APS) and dissolve it in 8ml of PBS to form an APS solution with a mass concentration of 10%;

[0050] 4) Add 50 μl of the APS solution obtained in step 3) to the mixture obtained in step 2), and mix it evenly;

[0051] 5) The mixture obtained in step 4) was sucked into a cylindrical silica gel mold with a diameter of 7.8mm and a height of 5.3mm in time, and placed in a freezer at -20°C for 18 hours to prepare a gelatin-sodium alginate porous cryogel.

[0052] According to the detection method described in Example 1, the samples obtained in Example 2 and Example 3 were tested respectively, and obtained respectively figure 2 , image 3 As shown in the ...

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Abstract

The invention discloses an injectable gelatin-sodium alginate porous cryogel with shape memory and a preparation method thereof. The invention uses natural gelatin and sodium alginate with good biocompatibility as raw materials, and prepares a gelatin-sodium alginate cryogel by low-temperature freezing polymerization. A new type of injectable cryogel material with shape memory function. By simulating the structure of natural extracellular matrix, the present invention improves the disadvantages of poor biocompatibility, poor mechanical properties and difficulty in injecting existing cryogels. And the pore penetration rate of the cryogel material prepared by this optimization method can reach 17%-43%, and the swelling rate in 2 hours can reach 7.4-10.6.

Description

technical field [0001] The invention belongs to the field of biomedical tissue engineering materials, in particular to an injectable gelatin-sodium alginate porous cryogel with shape memory and a preparation method thereof. Background technique [0002] Tissue engineering is to plant seed cells (cultured cells or stem cells) in an artificial extracellular matrix to form a structure, culture and expand in vitro, form a new tissue and then implant it into the patient's body to integrate with the tissue to regenerate and rebuild the tissue. The artificial three-dimensional scaffolds used in tissue engineering should simulate the composition and function of the extracellular matrix of natural tissues as much as possible. In tissue engineering strategies, injectable in situ hydrogels have the following advantages over preformed scaffolds: the initial sol / cell suspension can fill defects of any shape, and can be mixed with various therapeutic drugs for implantation. No need for s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08L89/00C08L5/04C08J9/28
CPCC08J9/28C08J2201/048C08J2389/00C08J2405/08
Inventor 肖文谦曲晓航谭云飞肖靖乐银鹏李波
Owner CHONGQING UNIVERSITY OF SCIENCE AND TECHNOLOGY