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An endophytic fungus that can enhance the photosynthesis of Schima superba
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A technology of endophytic fungi, photosynthesis, applied in the field of microorganisms
Active Publication Date: 2020-11-13
FUJIAN AGRI & FORESTRY UNIV
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At present, the report on the influence of endophytic fungi on the growth of Schima superba is still blank. In order to enrich the research on the influence of endophytic fungi on the growth of forest trees, the present invention screens out endophytic fungi that have a significant effect on the growth of Schima superba.
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Embodiment 1
[0011] Example 1 Isolation of endophytic fungi of Schima superba
[0012] 1. Main equipment
[0013] Ultra-clean workbench SW-CJ-1FD, constant temperature incubator HH•B11-II, constant temperature culture oscillator zhwy-211B, ten-thousandth balance AR1140, automatic vertical sterilizer LMQ.C-4060, ultrapure water Machine P60-CW etc.
[0014] 2. Main Reagents and Media
[0015] Reagents: 15% sodium hypochlorite, 75% absolute ethanol, primer PAGE 11-59bp OD 1-2, DNA electrophoresis loading buffer, GoodViewTM nucleic acid dye, 2×Tap PCR MasterMix, fungal DNA extraction kit, DNA purification and recovery kit.
[0016] Medium: (1) Modified Martin agar medium: peptone 5.0g, yeast extract powder 2.0g, glucose (C 6 h 12 o 6 •H 2 O) 20.0g, Potassium dihydrogen phosphate (KH 2 PO 4 ) 1.0 g, magnesium sulfate (MgSO 4 •7H 2 O) 0.5g, agar 15.0g, ultrapure water 1000ml, pH 6.2~6.6.
[0039] Bacterial solution preparation: Inoculate the activated endophytic fungi into 40 mL of modified Martin liquid medium, and culture them on a constant temperature shaker for 72 h (28 °C, 160 r min -1 ). Calculate and calculate the number of spores with the blood count method, and dilute the cultivated bacterial solution with sterile water to 5.5×10 6 pcs L -1 .
[0040]This experiment adopts soil culture pot experiment, and the seedlings of Schima superba selected for the experiment are 1-year-old seedlings, with an average seedling height of 20 cm and an average ground diameter of 3.0 mm, provided by Fujian Academy of Forestry Sciences. On March 3, 2016, Schima superba seedlings with consistent growth were selected and planted in plastic pots with a diameter of 15 cm and a height of 10 cm. The soil required for the test was mixed and weighed, and an equal amount (4kg) of red heart soil was placed in each pot. After a month of restorative growth, inoculation began on ...
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Abstract
The invention discloses an endophytic fungus capable of improving the photosynthesis of Schima superba. The endophytic fungus MJ49 is Fusarium ( Fusarium sp. ), which was registered and preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on April 8, 2019, with the preservation number CGMCC No.17478. The strain is isolated and purified from the stem of Schima superba, which can significantly improve the photosynthesis of Schima superba, thereby promoting plant growth.
Description
technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to an endophytic fungus capable of improving the photosynthesis of Schima superba. Background technique [0002] Schima superba is an important fire-resistant tree species in southern my country. It has the characteristics of high-quality growth promotion, good afforestation effect, and strong adaptability. It is one of the main timber forests in collective forest areas in southern my country. At present, there is still no report on the influence of endophytic fungi on the growth of Schima superba. In order to enrich the research on the influence of endophytic fungi on the growth of forest trees, the present invention screens out the endophytic fungi that can significantly promote the growth of Schima superba. Contents of the invention [0003] The object of the present invention is to provide an endophytic fungus capable of improving the photosynthesis of Schima superba....
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