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A kind of polyethylene plastic high-efficiency degrading bacteria and its separation and screening method and application

A polyethylene plastic and polyethylene technology, which is applied in the field of microorganisms, can solve the problems of insufficient effective bacterial species resources and low treatment efficiency, and achieve the effects of good degradation characteristics, convenient operation and broad application prospects.

Active Publication Date: 2019-11-26
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nowadays, there are few researches on biological treatment methods for plastic waste such as polyethylene, and there are not many effective strain resources, and the treatment efficiency is low, which limits its further expansion and application.

Method used

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  • A kind of polyethylene plastic high-efficiency degrading bacteria and its separation and screening method and application
  • A kind of polyethylene plastic high-efficiency degrading bacteria and its separation and screening method and application
  • A kind of polyethylene plastic high-efficiency degrading bacteria and its separation and screening method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Isolation, Screening and Identification of Polyethylene Degrading Bacteria

[0029] (1) Isolation and screening of polyethylene-degrading bacteria

[0030] Use the plastic waste floating from the port as the sample to be separated for separation and screening;

[0031] The collected plastic waste was washed with sterile water and cut into small pieces, placed in a sterile Erlenmeyer flask, added with 0.9% normal saline and glass beads, and placed in a constant temperature shaking incubator at 28 °C, 180 r / Incubate with shaking for 2 h to prepare a bacterial suspension. The bacterial suspension was inoculated with 2% ( v / v ) into a carbon-free mineral salt medium, adding UV-sterilized polyethylene powder (2 g / L), shaking culture at 28 °C and 180 r / min for 15 days, and setting up 3 groups in parallel. Regular sampling to determine the OD of the system 600 After the culture system became turbid, the transfer was continued, and the culture was repeated 3 times. Sel...

Embodiment 2

[0041] The degradation experiment of polyethylene film by polyethylene-degrading bacteria is carried out according to the following steps:

[0042] In order to evaluate the application potential of polyethylene-degrading bacteria in real life, polyethylene films common in daily life were selected for degradation experiments.

[0043] Inoculate MP-1 into LB medium with 2-5% inoculation amount and cultivate to logarithmic growth phase, wash 3 times with 0.01 mol / L phosphate buffer solution to remove the medium on the surface, and then use carbon-free minerals Resuspend in salt medium, then adjust the OD of the resuspended bacteria 600 The value is 1.0. Polyethylene film cut into 2×2 cm 2 After weighing, it was sterilized with 75% ethanol for 2 hours, and after taking it out, evaporate the ethanol on the surface with a sterile airflow in the ultra-clean bench. Add 90 mL of carbon-source-free mineral salt medium to the Erlenmeyer flask, add surface-sterilized polyethylene film ...

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Abstract

The invention relates to the field of microorganisms, in particular to a polyethylene plastic high-efficiency degrading bacteria and its separation and screening method and its application in the biodegradation of polyethylene plastics. The strain is preserved in the General Microbiology Center of the China Committee for the Preservation of Microorganisms, the preservation number is: CGMCCNo: 15724; the preservation time is: May 4, 2018. The strain has good environmental adaptability, has wide applications in the biodegradation of polyethylene plastics, especially polyethylene plastic films, provides new resources for the biodegradation of polyethylene plastics, and has good application prospects.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a polyethylene plastic high-efficiency degrading bacteria and its separation and screening method and its application in the biodegradation of polyethylene plastics. Background technique [0002] Plastics are organic compounds synthesized from petrochemical products. Due to their excellent chemical stability and mechanical properties, as well as their durability and economy, they are widely used in daily life. Plastic is hardly decomposed after disposal, and its continuous accumulation in the environment has caused serious ecological threats. "White pollution" has become a worldwide problem. Polyethylene (PE) is one of the synthetic polymers with the highest output and consumption in plastic products, and its global output is about 80 million tons per year. The basic structure of PE is -[CH 2 -CH 2 ] n -, due to its relatively large molecular weight, strong hydrophobicity, espec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02C02F3/34C12R1/01A62D101/20C02F101/32
CPCA62D3/02A62D2101/20C02F3/34C02F2101/32C12N1/20C12N1/205C12R2001/01
Inventor 涂晨刘颖周倩骆永明
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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