A strain of Trichosporum sinensis and its application
A Chinese technology of Mortierella and Mortierella, which is applied in the fields of application, method of using spores, plant cultivation, etc., and can solve problems not mentioned
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Embodiment 1
[0018] Example 1: Isolation and identification of Ophiocordyceps sinensis KD11-2 in China
[0019] 1. Acquisition of KD11-2 strain: Fresh Cordyceps sinensis was collected from Yala Township, Lucheng Town, Kangding City, Ganzi Prefecture, Sichuan Province, and transported to our laboratory through cryogenic preservation. In the aseptic operating room or ultra-clean workbench, peel off the soil and bacterial film around Cordyceps sinensis, disinfect the body surface with 75% alcohol; Part of it was cut into small pieces (pay attention to avoid the intestinal tract of the worm), planted on PPDA solid plate medium, and cultured at 9-16°C; the isolated typical colony of Cordyceps sinensis without pollution was confirmed as Cordyceps sinensis after molecular identification , to obtain the strain, and its number as KD11-2.
[0020] 2. Characteristics of KD11-2 strain:
[0021] Morphological characteristics: On the PPDA solid medium, the colony is earthy yellow, the surface of the c...
Embodiment 2
[0028] Experimental location: Guangdong Institute of Applied Bioresources located in Guangzhou City, the following cultivations are all under normal oxygen concentration.
[0029]Inoculate the Mortierella chinensis KD11-2 strain and other Mortierella chinensis strains (KD1223) into sterile solid PPDA medium respectively, and after culturing in the dark at 9°C for 60 days, pick colonies and inoculate them into sterile liquid PPDA medium , at 9°C, 100rpm shaking culture (dark culture) for 65 days, select the fermentation culture of mycelial balls with uniform size, diameter of 2-3 mm, and light brown bacterial liquid as a liquid strain, which is used for the production of fruiting bodies of Cordyceps sinensis Cultivated production.
[0030] In a sterile room or an ultra-clean workbench, the liquid strain diluted 5 times with sterile water (according to every 100g of the cultivation medium was added 30mL of the diluted liquid strain) was inserted into the sterile cultivation medi...
Embodiment 3
[0033] Experimental location: Guangdong Institute of Applied Bioresources located in Guangzhou City, the following cultivations are all under normal oxygen concentration.
[0034] The Mortierella sinensis KD11-2 strain and other Mortierella sinensis strains (KD1223) were inserted into sterile solid PPDA medium respectively, and after dark culture at 16°C for 45 days, the colonies were selected and placed into sterile liquid PPDA medium. Under 16°C, 100rpm shaking culture (dark culture) for 40 days, select the fermentation culture with uniform mycelial ball size and a diameter of 2-3 mm as a liquid strain for the cultivation and production of fruiting bodies of Cordyceps sinensis.
[0035] In a sterile room or ultra-clean workbench, inject 10-fold liquid strains diluted with sterile water (according to every 100 g of cultivation medium, insert 30 mL of diluted liquid strains) into the sterile cultivation medium. Place the inoculated culture bottle at 13°C for dark culture for 4...
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