A kind of preparation method of vanadium oxide and its application
A technology of vanadium oxide and vanadium oxide quantum, which is applied in the preparation of nanomaterials and the fields of antibacterial and detection and sensing, can solve the problems of high cost, cumbersome and time-consuming steps, and long synthesis cycle, and achieve enhanced antibacterial performance, remarkable antibacterial effect, Wide range of antibacterial effects
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Embodiment 1
[0016] S1. Weigh 0.2g of vanadium trichloride in 20mL of ethanol, fully stir and dissolve;
[0017] S2. Transfer the solution prepared in step S1 into a polytetrafluoroethylene sealed tank and put it into a hydrothermal reaction kettle, and heat it at a temperature of 180° C. for 10 hours;
[0018] S3. After the water-heated solution drops to room temperature, take out the polytetrafluoroethylene tank, take out the solution into a centrifuge tube, centrifuge at a speed of 12000 rpm for 10 minutes, and take the supernatant as the prepared vanadium oxide Quantum dot solution;
[0019] S4. When conducting antibacterial experiments, it is necessary to take an appropriate volume of vanadium oxide quantum dot solution, place it in a constant temperature drying oven and heat it at 50°C until the ethanol is completely evaporated, then add an equal volume of distilled water to obtain a vanadium oxide quantum dot aqueous suspension .
Embodiment 2
[0021] In this embodiment, the vanadium oxide quantum dot solution prepared in Example 1 is applied to the detection of hydrogen peroxide, and 10 μL TMB (20 mM), 30 μL VO x QD (10mg mL -1 ) and various concentrations of H 2 o 2 Add to acetate buffer (200mM, pH=3) to achieve a total volume of 200 μL of the mixed solution, react at 40°C for 30 minutes, use a microplate reader to measure the absorbance of the solution at 652nm, blank control experiment Use PBS (without H 2 o 2 ) solution, the measurement results are as figure 2 As shown in (A), the detection linear range of vanadium oxide quantum dots to hydrogen peroxide is 0.5-100μM, and the regression equation y=0.00713x+0.18125(R 2 = 0.9923).
[0022] In this example, the vanadium oxide quantum dot solution prepared in Example 1 is applied to the detection of glucose, and glucose with different final concentrations (0.005-4mM) is added to the solution containing glucose oxidase (2mg mL -1 ) in PBS (pH 7.4), first incu...
Embodiment 3
[0024] In this embodiment, the vanadium oxide quantum dot solution prepared in Example 1 is applied to the antibacterial experiment in vitro, and the single colony non-drug-resistant and drug-resistant bacteria on the solid LB medium are inoculated into 50mL sterile liquid LB medium [containing tryptone (0.5g), yeast extract (0.25g) and NaCl (0.5g)], then the suspension of non-resistant and resistant bacteria was placed on a rotary shaker at 180 rpm at 37°C overnight . Bacteria were subsequently diluted to 10 with sterile PBS 6 CFU mL -1 , the obtained bacterial solution (200 μL) was mixed with 1 mgmL -1 VO x QD and 50 μM H 2 o 2 Incubate at 37°C for 30 minutes, then incubate the solution on solid medium at 37°C for 24 hours, count the number of bacterial colonies by the CFU method, use PBS as a blank control, bacteria alone and H 2 o 2 or VO x QD carried out parallel control experiments, and the measurement results were as follows: image 3 As shown in (A), add H at ...
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Abstract
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