Isolated frog heart perfusion experimental teaching device

Abstract

The invention discloses an isolated frog heart perfusion experimental teaching device. The device comprises a liquid storage tank, an arterial cannula head and a fixed rod; a side pipe is arranged atan outside of the top end of the liquid storage tank, the side pipe is plugged in one end of the fixed rod, a lower end of the liquid storage tank is plugged in the arterial canula head, and one sidehook and a liquid adding pipe are arranged on the sidewall of the arterial cannula head. The device disclosed by the invention has the features of being easy to intubate, preventive in coagulation, easy to fix, and convenient to change the liquid; the successful rate of manufacturing the isolated heart perfusion sample can be greatly improved, and the stability and reliability of the systole function detection experimental data are improved.

Description

technical field [0001] The invention relates to a device for teaching experiments related to life sciences, in particular to a teaching device for perfusion experiments of isolated frog hearts. Background technique [0002] Using the isolated heart perfusion system can visually observe the activity of the heart, study the systolic function of the heart, the change of heart rate and rhythm, and the influence of various body fluid factors on the heart function. It is one of the important means to study the heart function and is widely used in medicine. , biology, pharmacy and other teaching and research. The single-tube method (Straub’s method) cardiac perfusion experiment using the isolated frog heart has simple requirements for the experimental environment. The whole experimental process does not require constant temperature and oxygen supply equipment, and is widely used in life science experiment teaching. However, due to the structural characteristics of the frog-heart i...

AI Technical Summary

Problems solved by technology

However, the traditional single-tube method has many disadvantages in practical application due to the structural characteristics of frog-heart intubation and frog-heart tube clips.

Because the frog heart cannula is made of glass, it is easily damaged during the experiment; compared with the frog aorta, the tip of the frog heart cannula is thicker and blunt, which makes it difficult to insert into the ventricle, making it difficult to make cardiac perfusion specimens; it is inconvenient and easy to flush the blood in the cannula during intubation. Cause blood coagulation blockage, increase the chance of intubation failure; because the clamp of the frog heart tube is not firmly clamped, it is easy to affect the measurement of cardiac contraction tension during the experiment; during the experiment, it is necessary to use a straw to add or extract the liquid in the arterial cannula, which is easy to touch. The inner wall of the tube affects the accuracy and reliability of the experimental data

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