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Breeding method and application of a kind of high oleic acid rapeseed male sterile line

A technology of nuclear sterile line and high oleic acid, which is applied in the field of rapeseed breeding, to achieve the effect of increasing market value and supply, reducing production costs, and improving economic benefits and market competitiveness of enterprises

Active Publication Date: 2020-04-28
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the development of gene-specific markers targeting these two loci and their effective use in high oleic male sterile lines has not been reported

Method used

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  • Breeding method and application of a kind of high oleic acid rapeseed male sterile line
  • Breeding method and application of a kind of high oleic acid rapeseed male sterile line
  • Breeding method and application of a kind of high oleic acid rapeseed male sterile line

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Development and Verification of Molecular Markers

[0026] 1. Development and verification of Ms3 / ms3 markers

[0027] In this example, the gene sequence at the Bnms3 locus and the sequence between the corresponding wild-type BnC9.Tic40 and the homologous gene BnA10.Tic40 were analyzed. See figure 1 shown, where Indicates the start position and end position of the primer; → indicates the sequence of m3c9-4F / 2R; → indicates the sequence of M3C9-1F / 1R. Aiming at the difference of Bnms3 at 1200 to 1222 and the difference of BnC9.Tic40 at 1630 to 2050, an ARMS-PCR allele-specific marker M3C9-1F / 1R and m3c9-4F / 2R dual PCR system was developed, and the primer sequences are as follows :

[0028] M3C9-1F: 5'-CTGAAGCTTCTTTAACTGTATGATGC-3', see SEQ ID NO: 1;

[0029] M3C9-1R: 5'-TAGAGGGAACAATCTAGATGAG-3', see SEQ ID NO:2;

[0030] m3c9-4F: 5'-GGACATGTTTTTGTTTTTTGG-3', SEQ ID NO: 3;

[0031] m3c9-2R: 5'-AGGTTGTGGCTTAGACACAGGAG-3', SEQ ID NO:4.

[0032] The PCR sy...

Embodiment 2

[0053] Example 2 Breeding of GMS Lines of High Oleic Rapeseed

[0054] 1. See the breeding route of the present invention Figure 5 . The target genotype identified by prospect selection in the breeding roadmap of the present invention is ms3ms3ms4 with high oleic acid b ms4 b (homozygous sterile strain A) and Ms3ms3ms4 b ms4 b (maintaining strain B) and ms3ms3ms4 c ms4 c (temporary maintenance strain C). The background selection is based on the results of the foreground selection. The selected individual plants with consistent growth and shape are analyzed using the 6K breeding chip (produced by Zhongyujin Company) markers. The recurrent parents are used as a reference, and the genetic similarity of the three ABC lines is compared at the same time. Individual plants with better similarity enter into the next generation. In the present invention, the non-transgenic high oleic acid sterility material with a stable oleic acid content greater than 75% is finally bred thro...

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Abstract

The invention belongs to the technical field of oilseed rape breeding, and particularly relates to a breeding method of a genic male sterility line of oilseed rape with high oleic acid and an application of the breeding method. According to the method, a cabbage type rape recessive epistasis interacting nucleus male sterile complete sterile line 14A4028-1 (with genotype of ms3ms3ms4ms4<c>) withcommon oleic acid content lower than 69% is hybridized with an oilseed rape material with high oleic acid, oleic acid content of seeds of the oilseed rape material is higher than 75%, rapid and accurate genotype identification is performed by a newly developed molecular marker, and a high-oleic acid genic male sterility line material with oleic acid content higher than 75% is cultured by filial generations obtained by backcross transformation of the material through combination of auxiliary methods of gas chromatography and the like. By means of the molecular marker and the breeding method thereof, the single plant can be effectively selected according to the genotype at any stage of the breeding process, the selection efficiency is improved, and the breeding cycle is shortened.

Description

technical field [0001] The invention belongs to the technical field of rapeseed breeding, and in particular relates to a method for breeding a high-oleic-acid rapeseed male sterile line and its application. Background technique [0002] Rapeseed oil content is a quantitative trait regulated by various fatty acid pathways, and oil content is significantly positively correlated with oleic acid content. Oleic acid is a natural unsaturated fatty acid with high nutritional value and good thermal stability. In terms of consumption, oleic acid can reduce the concentration of low-density lipoprotein in human blood, which is beneficial to human cardiovascular. In industry, high oleic acid oil can be effectively methylated, and the quality of biodiesel produced is better. [0003] 9012A is a nuclear sterile type discovered by Chen Fengxiang, which can produce all sterile populations, which solves the dilemma of eliminating 50% of fertile plants in production. The current study cons...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04C12Q1/6895
CPCA01H1/02A01H1/04C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 周永明李旭张莉莉范楚川黄会斌
Owner HUAZHONG AGRI UNIV