Microfluidic chip box for full-automatic nucleic acid extraction and amplification detection and application thereof

A microfluidic chip and fully automatic technology, applied in the field of nucleic acid detection in molecular biology, can solve problems such as inability to meet rapid on-site detection, and achieve the effect of avoiding pollution problems and avoiding cross-contamination

Pending Publication Date: 2019-10-15
宁波迪亚生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the problem of sample storage and detection timeliness, there is an urgent need for rapid detection in these fields. The traditional laboratory operation pr

Method used

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  • Microfluidic chip box for full-automatic nucleic acid extraction and amplification detection and application thereof
  • Microfluidic chip box for full-automatic nucleic acid extraction and amplification detection and application thereof
  • Microfluidic chip box for full-automatic nucleic acid extraction and amplification detection and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0043] Example 1 Preparation of a microfluidic chip box for automatic nucleic acid extraction, amplification and detection of this application

[0044] The microfluidic chip box of this application is prepared using engineering plastics such as polypropylene or polycarbonate as the main body. Image 6 As shown, the structure is Figure 1-5 Shown, including waste liquid storage negative pressure pipe; waste liquid suction port; waste liquid pipe; filling port; lysis chamber; suction elution port; elution negative pressure pipe; pumping reaction pool port; buffer storage; reaction pool negative pressure pipe ; Nucleic acid amplification detection chamber fixed card; reaction pool; reaction pool switch; mixing tube; reagent chamber; elution tube; nucleic acid extraction membrane; lysis chamber switch; waste liquid chamber; rubber plug; isolation membrane; chip box main body; nucleic acid expansion Increase and test chamber body; fill cover; reagent placement port; isolation cover.

Example Embodiment

[0045] Example 2 Use the present application for the fully automated nucleic acid extraction and amplification detection microfluidic chip box for HIV-1, HBV, and HCV virus triple detection

[0046] The whole set of automatic PCR tester is self-made by our company, including fluorescence detection unit, temperature control unit, automatic control unit, sensing and data analysis, display unit, automatic nucleic acid extraction and amplification detection microfluidic chip box and other parts. Among them, the main function of the fluorescence detection unit is to perform real-time fluorescence quantitative detection of the test sample during the PCR reaction; the temperature control unit realizes precise control of the nucleic acid extraction and temperature changes in the PCR reaction; the automatic control unit is responsible for the amount of reagents in the nucleic acid extraction reaction Precise control; the main function of the sensor, data analysis, and display unit is to co...

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Abstract

The invention provides a microfluidic chip box for full-automatic nucleic acid extraction and amplification detection, and a detection method and an application thereof. The microfluidic chip box is composed of a chip box main body, a nucleic acid amplification detection bin body and a filling cover, wherein the chip box main body comprises a waste liquid bin, a cracking bin and a reagent bin; thenucleic acid amplification detection bin body comprises a reaction pool, and a mixing pipe and a reaction pool negative pressure pipe which are connected with the reaction pool; the filling cover isarranged on the cracking bin, and more than one liquid filling hole is formed in the filling cover; a nucleic acid extraction membrane separating the cracking bin into two parts is arranged in the cracking bin; parts, that are not provided with the filling cover, of the two parts of the cracking bin communicate with the reagent bin through elution pipes and communicate with the waste liquid bin through waste liquid pipes, and the reagent bin communicates with the reaction pool through the mixing pipe. The microfluidic chip box for full-automatic nucleic acid extraction and amplification detection can efficiently complete the steps of nucleic acid extraction, reagent mixing and the like, and meet the needs of nucleic acid amplification detection.

Description

technical field [0001] The invention belongs to the field of nucleic acid detection in molecular biology. Specifically, the invention provides a microfluidic chip box for automatic nucleic acid extraction and amplification detection, which can realize the "sample-result" combination in cooperation with corresponding automatic fluorescence detection instruments. Rapid automated nucleic acid molecular detection. Background technique [0002] Nucleic acid molecular detection has been widely used in pathogen detection, genetic disease prevention and control, variety identification, pollution monitoring and other inspection fields. Due to the problem of sample storage and detection timeliness, there is an urgent need for rapid detection in these fields. The traditional laboratory operation process of sample processing-nucleic acid extraction-nucleic acid amplification-nucleic acid amplification obviously cannot meet the needs of rapid on-site detection. [0003] The microfluidic...

Claims

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Application Information

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IPC IPC(8): C12M1/38C12M1/34C12M1/00C12Q1/70C12Q1/686C12Q1/6806C12R1/93
CPCC12Q1/703C12Q1/706C12Q1/707C12Q1/686C12Q1/6806C12Q2600/16C12Q2565/629C12Q2561/101C12Q2537/143C12Q2523/308Y02A50/30
Inventor 王书琦李玉峰
Owner 宁波迪亚生物科技有限公司
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