Integrated device with biological living tissue 3D printing function and tissue cell culturing function

Abstract

Provided is an integrated device with a biological living tissue 3D printing function and a tissue cell culturing function. A bionic tissue engineering incubator is provided with a liquid inlet and aliquid outlet. A lifting platform for bearing printed tissue is arranged in a cavity of the incubator. A 3D printing device is located on the incubator. The free end of each mechanical arm of the printing device is provided with a printing nozzle. An internal thread sleeve is fixed at the lower end of the lifting platform and is in sliding fit with a sealing element in an axial hole in the bottomof the incubator. A driving motor is located below the incubator. A rotating shaft of the driving motor is matched with the internal thread sleeve through a screw. A guide slide rail is arranged in the incubator. The lifting platform is in sliding fit with the guide slide rail. The 3D printing device, the driving motor and a heater are all controlled by a computer software control system. The driving motor is controlled by the computer software control system to drive the lifting platform to gradually descend in the 3D printing process, so a printed tissue gray body descends to be soaked in aculture solution in the incubator, and the printing plane is kept above the liquid surface of the culture solution.

Description

technical field [0001] The invention relates to the field of biological tissue engineering preparation devices, in particular to a device integrating 3D printing of living tissue and tissue cell culture. Background technique [0002] 3D bioprinting is a method for constructing engineered human tissue with good application prospects. With the rapid development of 3D bioprinting technology in recent years and the continuous improvement of people's requirements for precision medicine, 3D bioprinting has unique personalized characteristics. , has drawn more and more people's attention. At present, my country has listed 3D printing technology as the focus of technological development. [0003] Since biological 3D printing technology has the characteristics of cell-containing printing, it can only use a curing process with a high degree of cell compatibility. At present, ultraviolet curing molding technology is mainly used. Due to the poor penetration of ultraviolet light, the p...

AI Technical Summary

Problems solved by technology

However, low temperature often leads to damage to the mitochondria of cells, making it difficult to maintain a high level of cell activity, and it is impossible to achieve high-activity bio-3D printing, which severely limits the clinical application of bio-3D printing.

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