Method for isolating rice Torrubiella dabieshanensis and method for preparing artificial inoculum thereof

Abstract

The invention discloses a method for isolating rice Torrubiella dabieshanensis and a method for preparing an artificial inoculum thereof. The problem that the northern disease sample has a low successrate of isolation, and the strain isolated by the chlamydospore shaking method of the sample cannot be determined as a monosporous strain, disease specimens are accompanied by a large number of parasitic saprophytic germs, target bacteria grow slowly and isolation is difficult is solved in the invention. The method of the invention includes the following steps: (1) Selection of rice Torrubiella dabieshanensis samples: re-collected samples are preserved for no longer than 9 months; re-collected rice Torrubiella dabieshanensis samples are fresh and white, yellow or a similar color; 2) preparation of a chlamydospore suspension; (3) diluting of the chlamydospore suspension prepared in Step (2); (4) coating of the chlamydospore suspension diluted in Step (3); and (5) isolation of rice Torrubiella dabieshanensis after coating. By the method, the target monosporous strain can be isolated and purified within 3 days, and the artificial inoculums can be prepared within 7 days. The method has the best isolation effect on freshly harvested rice Torrubiella dabieshanensis, and has a good application and promotion prospect.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a high-efficiency and rapid separation method of Ustilaginoidea albicans and a preparation method of an artificial inoculum. Background technique [0002] Ustilaginoidea albicans is a fungal disease on the ear of rice, belonging to Ascomycetes and Ergotaceae. Since the late 1990s, with the adjustment of agricultural planting structure, the large-scale promotion of high-quality rice and hybrid rice, especially the two-line hybrid rice, the increase in the use of nitrogen fertilizers and the accumulation of pathogenic bacteria, the occurrence of Chrysanthemum albicans has increased. trend. Chlorosclerotinum oryzae is a new disease discovered in recent years that can infect rice ears. Before 2011, C. oryzae was found in rice fields in Liaoning Province. With the progress of related research on C. oryzae, it was only found in paddy fields in Zhejiang, Shaanxi and other provinces in 201...

AI Technical Summary

Problems solved by technology

[0004] The technical problem solved by the present invention is that the success rate of isolation of northern disease samples is low, the bacterial strains isolated by the sample chlamydospore shake-off method cannot be determined to be monospore strains, the disease samples are accompanied by a large number of parasitic saprophytic miscellaneous bacteria, and the target bacteria grow slowly , separation difficulties, etc., provide a method for isolating Chlorococcus oryzae and its artificial inoculum that can efficiently and quickly isolate the target pathogen from disease samples in a short period of time

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