Preparation method and application of inverse opal structure gel membrane for detecting alpha-fetoprotein
An anti-opal structure and alpha-fetoprotein technology, applied in the field of material chemistry and analysis and detection, can solve the problems of cumbersome preparation steps, complicated operation, time-consuming and labor-consuming, etc., and achieve good reusability, simple preparation method and convenient operation Effect
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Embodiment 1
[0044] The specific preparation steps of the inverse opal structure gel film for detecting alpha-fetoprotein of the present invention are as follows:
[0045] 1) ultrasonically dispersing monodisperse silica microspheres into n-butanol solution, wherein the mass fraction of silica is 0.4%, to prepare silica colloidal solution;
[0046] 2) Clean the silicon wafer with deionized water, soak it in piranha solution (a mixed solution of 98% concentrated sulfuric acid and 30% hydrogen peroxide, volume ratio 7:3) for 10 hours to remove surface impurities, and then clean it with pure water , dried with nitrogen to obtain a hydrophilic matrix;
[0047] 3) Place the hydrophilized substrate silicon chip in a watch glass, inject pure water and the colloidal silica solution of step 1) in sequence, keep the gap between the water and the silicon chip at 2 mm, and use the surface tension to make the two Disperse silicon oxide evenly on the surface of water, let it stand at room temperature f...
Embodiment 2
[0055] The specific preparation steps of the inverse opal photonic crystal film for detecting α-fetoprotein of the present invention are as follows:
[0056] 1) ultrasonically dispersing monodisperse silica microspheres into n-butanol solution, wherein the mass fraction of silica is 0.4%, to prepare silica colloidal solution;
[0057] 2) Clean the silicon wafer with deionized water, soak it in piranha solution (a mixed solution of 98% concentrated sulfuric acid and 30% hydrogen peroxide, volume ratio 7:3) for 10 hours to remove surface impurities, and then clean it with pure water , dried with nitrogen to obtain a hydrophilic matrix;
[0058] 3) Place the hydrophilized substrate silicon chip in a watch glass, inject pure water and the colloidal silica solution of step 1) in sequence, keep the gap between the water and the silicon chip at 3 mm, and use the surface tension to make the two Disperse silicon oxide evenly on the surface of water, let it stand at room temperature fo...
Embodiment 3
[0066] With embodiment 1 ( figure 1 a) and Example 2 ( figure 1 b) The prepared inverse opal structure gel membrane for detecting α-fetoprotein was observed under a scanning electron microscope, and it can be seen that it is obviously porous, the pore size is uniform, the size of the gap is about 200nm, and there is no obvious structural difference, Specific scanning electron microscope photos such as figure 1 shown.
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