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Method for controlling heterozygous algae in large-area culture pool of salt algae

A cultivation pond and large-area technology, applied in the field of algae cultivation, can solve the problems of damage to salina, the cultivation environment is easily affected by external influences, and the growth and reproduction of salina, etc., and achieve the effect of improving the utilization rate.

Active Publication Date: 2019-11-12
HEXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the process of large-scale cultivation of salina, because its cultivation environment is easily affected by the outside world, it is extremely vulnerable to Dunaliella microella, Chlorella, Nannochloropsis and Cryptorodgiella salina, which are widely distributed and have poor stress resistance. Strong algae pollution, which will affect the growth and reproduction of salina itself, and then affect the content of β-carotene and the quality of salina algae powder
Even with the continuous improvement of breeding technology and the gradual expansion of indoor breeding scale, algae pollution cannot be completely avoided
At present, copper sulfate drugs are mostly used to remove algae, which will also cause damage to salina algae while killing algae, and a lot of work is required to purify the water that has been added with drugs.

Method used

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  • Method for controlling heterozygous algae in large-area culture pool of salt algae
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A method for controlling miscellaneous algae in large-area salina culture ponds, comprising the steps of:

[0037] S1: Add water to 1 / 3 volume in the culture tank in advance, insert the pre-cultured algae into the culture tank, adjust the water temperature to 47°C, keep it for 5 hours after the water temperature rises, and then cool down to 42°C;

[0038] S2: Continuously provide 20,000 lx of light to the culture pond for the first stage of irradiation for 10 hours;

[0039] S3: After the irradiation, stop the light for 1 hour, replenish the water to the initial water volume, and provide more than 20000 lx of light again, and carry out the second stage of light for 5 hours;

[0040] S4: repeat the operation of step S3 3 times;

[0041] S5: After the light is over, reduce the light intensity to 15000lx, replenish the water to the initial water volume, and lower the water temperature to 40°C;

[0042] S6: adding a mixture of citric acid and tartaric acid with a molar ra...

Embodiment 2

[0046] A method for controlling miscellaneous algae in large-area salina culture ponds, comprising the steps of:

[0047] S1: Add water to 1 / 2 volume in the culture tank in advance, insert the pre-cultured algae into the culture tank, adjust the water temperature to 50°C, keep it for 2 hours after the water temperature rises, and then cool down to 42°C;

[0048] S2: Continuously provide 15000lx of light to the culture pond for the first stage of irradiation for 18 hours;

[0049] S3: After the irradiation is over, stop the light for 0.5 hours, replenish the water to the initial water volume, and provide more than 15000 lx of light again, and carry out the second stage of light for 5.5 hours;

[0050] S4: repeat the operation of step S3 3 times;

[0051]S5: After the light is over, reduce the light intensity to 10000lx, replenish the water to the initial water volume, and lower the water temperature to 40°C;

[0052] S6: adding a mixture of citric acid, fumaric acid and tarta...

Embodiment 3

[0056] A method for controlling miscellaneous algae in large-area salina culture ponds, comprising the steps of:

[0057] S1: Add water to 1 / 3 volume in the culture tank in advance, insert the pre-cultured algae into the culture tank, adjust the water temperature to 49°C, keep it for 3 hours after the water temperature rises, and then cool down to 44°C;

[0058] S2: Continuously provide 18000lx of light to the culture pond for the first stage of irradiation for 12 hours;

[0059] S3: After the irradiation, stop the light for 1 hour, replenish the water to the initial water volume, and provide more than 16000lx of light again, and carry out the second stage of light for 5 hours;

[0060] S4: repeat the operation of step S3 3 times;

[0061] S5: After the light is over, reduce the light intensity to 12000lx, replenish the water to the initial water volume, and lower the water temperature to 40°C;

[0062] S6: adding a mixture of citric acid, fumaric acid and tartaric acid in a...

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Abstract

The invention provides a method for controlling heterozygous algae in a large-area culture pool of salt algae. A series of adversity conditions such as high temperature, long-time strong lighting, acid environment and high salt environment are sequentially and artificially created, the physiological activity of the heterozygous algae is gradually weakened, and even killed, while the salt algae cansurvive from the superior adversity resistance of the salt algae, meanwhile acid substances and salts added in the acid and high-salt environment can directly provide nutrients for the growth and reproduction of the salt algae after dilution, and the situation of killing two birds with one stone is achieved. The method can effectively inhibit the heterozygous algae without affecting the activityof the salt algae, and the utilization rate of various additives can be effectively increased, and the method is suitable for wide application in large indoor culture pools.

Description

technical field [0001] The invention belongs to the technical field of algae cultivation, and in particular relates to a method for controlling miscellaneous algae in large-area salina cultivation ponds, which is suitable for indoor cultivation ponds. Background technique [0002] Salina, also known as Dunalsel algae (Dunalselal.salana), is a halophilic single-celled algae belonging to the Chlorophyta. Carotenoid is an important food coloring, which widely exists in plants, algae and fungi. At present, it has been found that β-carotene has a strong function of scavenging free radicals, and it is beneficial to the prevention and treatment of tumors, cardiovascular and cerebrovascular diseases. Disease, Alzheimer's disease, and age-related degenerative diseases such as cataracts have a certain role, so it has become a hot health product, and it is also often used to protect eyesight and relieve eye fatigue. However, industrially synthesized β-carotene is mostly in the trans s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N1/38C12R1/89
CPCC12N1/12C12N1/38
Inventor 罗光宏杨宋琪王丽娟杨生辉祖廷勋徐卫明王丹霞陈天仁
Owner HEXI UNIV