Method of detecting Salmonella typhimurium
A technology for Salmonella and typhoid, which is applied in the field of analysis and detection, can solve the problems that hinder the application of immunochromatography technology and low sensitivity of immunochromatography technology, and achieve the effect of rapid on-site detection, good specificity and low detection limit
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Embodiment 1
[0020] A method for detecting Salmonella typhimurium, comprising the following steps:
[0021] (1) Preparation and modification of magnetic upconversion nanoparticles:
[0022] Fe 3 o 4 Preparation of nanoparticles: Accurately weigh 11.2 mM ferric chloride solution and 5.6 mM ferrous chloride solution, add them into 180mL deionized water, stir well until completely dissolved. Then heated to 50°C under nitrogen protection, the mixed solution was mixed with 12.5 mL 0.1M NH 3 ·H 2 The O solution was mixed and stirred vigorously for 30 min. Under the action of an external magnetic field, wash with absolute ethanol and deionized water alternately until neutral, redissolve in aqueous solution, and store at 4°C for later use;
[0023] Fe 3 o 4 Preparation of @UCNPs nanoparticles: firstly, the corresponding trifluoroacetate was prepared by dissolving the corresponding rare earth oxide in a trifluoroacetic acid solution, refluxing at 80 °C for 24 h, and drying at 60 °C. The...
Embodiment 2
[0032] A method for detecting Salmonella typhimurium, comprising the following steps:
[0033] (1) Preparation and modification of magnetic upconversion nanoparticles:
[0034] Fe 3 o 4 Preparation of nanoparticles: Accurately weigh 11.2 mM ferric chloride solution and 5.6 mM ferrous chloride solution, add them into 180mL deionized water, stir well until completely dissolved. Then heated to 50°C under nitrogen protection, the mixed solution was mixed with 12.5 mL 0.1M NH 3 ·H 2 The O solution was mixed and stirred vigorously for 30 min. Under the action of an external magnetic field, wash with absolute ethanol and deionized water alternately until neutral, redissolve in aqueous solution, and store at 4°C for later use;
[0035] Fe 3 o 4 Preparation of @UCNPs nanoparticles: firstly, the corresponding trifluoroacetate was prepared by dissolving the corresponding rare earth oxide in a trifluoroacetic acid solution, refluxing at 80 °C for 24 h, and drying at 60 °C. The...
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