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Method for determining human serum folic acid

A serum folic acid and determination method technology, applied to the measurement of scattering characteristics, etc., can solve the problems of complex sample processing process, easy oxidation of folic acid, high background, etc., and achieve the effect of low price, high sensitivity, and elimination of interference

Inactive Publication Date: 2019-11-29
NINGBO AUCHEER BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, expensive precision instruments and reagents are required, and the sample processing process is relatively complicated; in addition, the results of the enzyme-linked immunoassay are quite different from the actual values.
The microbial method can sensitively detect bioactive folic acid, which is the biggest feature and advantage of the microbial method compared with other methods, but the traditional microbial method also has some defects: for example, folic acid is easily oxidized, and the background is relatively high during detection

Method used

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  • Method for determining human serum folic acid
  • Method for determining human serum folic acid
  • Method for determining human serum folic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A method for assaying human serum folic acid, comprising the steps of:

[0017] (1) Preparation of experimental water: add 4 g of activated carbon powder to 1 L of deionized water, stir for 30 s, and remove the activated carbon powder by suction filtration with a G4 sand core funnel to obtain experimental water;

[0018] (2) Preparation of folic acid standard solution: Weigh 200mg of folic acid standard product, dissolve it with 0.01mol / L ammonia water and dilute to 1L, absorb 1mL of the above solution, then dissolve it with 0.01mol / L ammonia water and dilute to 1L, and then absorb 1mL , dissolved with 0.01mol / L ammonia water and fixed to 25mL; adjusted to zero with 0.01mol / L ammonia water, the optical path of the cuvette is 1cm, the colorimetric wavelength is 256nm, according to the UV absorbance value, calculate the folic acid concentration of the solution, and then according to the actual Measure the concentration and dilute it with 0.5% sodium ascorbate solution to ...

Embodiment 2

[0024] A method for assaying human serum folic acid, comprising the steps of:

[0025] (1) Preparation of experimental water: add 4 g of activated carbon powder to 1 L of deionized water, stir for 30 s, and remove the activated carbon powder by suction filtration with a G4 sand core funnel to obtain experimental water;

[0026] (2) Preparation of folic acid standard solution: Weigh 200mg of folic acid standard product, dissolve it with 0.01mol / L ammonia water and dilute to 1L, absorb 1mL of the above solution, then dissolve it with 0.01mol / L ammonia water and dilute to 1L, and then absorb 1mL , dissolved with 0.01mol / L ammonia water and fixed to 25mL; adjusted to zero with 0.01mol / L ammonia water, the optical path of the cuvette is 1cm, the colorimetric wavelength is 256nm, according to the UV absorbance value, calculate the folic acid concentration of the solution, and then according to the actual Measure the concentration and dilute it with 0.5% sodium ascorbate solution to ...

Embodiment 3

[0032] Get the serum samples (24ug / L, 12ug / L, 6ug / L) of 3 kinds of folic acid concentrations of high, medium and low, embodiment 1 measures 10 times continuously with this method, see table 1, the average value of measuring is respectively 23.65, 11.95, 5.96ug / L, the intra-assay coefficient of variation is 2.3%, 2.9%, 3.9%; Example 2 is continuously measured 10 times by this method, see Table 2, and the average values ​​measured are respectively 23.6, 11.97, 5.94ug / L , the intra-assay coefficient of variation is 2.5%, 2.5%, 4.2%;

[0033] Table 1

[0034] high concentration sample medium concentration sample Low concentration samples the first time 23.6 12.2 6.5 the second time 23.2 12.3 5.9 the third time 23.4 11.2 5.2 the fourth time 23.5 11.6 6.2 the fifth time 22.9 12.1 6.7 the sixth time 24.3 11.7 6.1 Seventh time 24.1 11.8 5.2 The eighth time 23.5 12.1 5.6 ninth time 24.8 12.2 5.1 ...

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Abstract

The invention discloses a method for determining human serum folic acid. The method comprises the steps of preparation of experimental water, preparation of a folic acid standard solution, preparation of a lactobacillus rhamnosus growth medium, preparation of lactobacillus rhamnosus, preparation of a detection medium and detection of the serum folic acid. By adopting activated carbon to adsorb organic impurities in water, the purpose of eliminating interferences can be achieved; the folic acid is unstable in chemical property and easily oxidized; ascorbic acid is added in the growth and detection mediums as an antioxidant, so that the oxygen in dissolved blood is reduced as much as possible, so that the content of deoxyhemoglobin is increased, and the effect of protecting the folic acid is achieved; and during detection, a cell culture plate is sealed by a sealing film, so that growth of the lactobacillus rhamnosus is facilitated, and moisture evaporation and oxidation of the folic acid can also be prevented. The method for determining the human serum folic acid provided by the invention is simple and easy to implement, low in price, high in sensitivity, and is more suitable for the folic acid screening needs of large-scale population.

Description

technical field [0001] The invention relates to the field of in vitro diagnosis, in particular to a method for measuring human serum folic acid. Background technique [0002] Folic acid is an important nutrient needed by the body. It is a water-soluble vitamin and participates in the synthesis of amino acids, purines, and pyrimidines in the body. At the earliest, folic acid was mainly used clinically to treat and prevent megaloblastic anemia and prevent embryonic neural tube defects. At present, there are mainly two types of folic acid detection samples commonly used in clinical practice: serum folic acid detection and red blood cell folic acid detection. Serum folic acid level is an index reflecting recent folic acid intake, and it can also be used clinically for the etiological diagnosis of cellular anemia. Red blood cell folic acid can best reflect the long-term storage of folic acid, and low red blood cell folic acid indicates long-term folic acid deficiency. [0003]...

Claims

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Application Information

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IPC IPC(8): G01N21/51
CPCG01N21/51
Inventor 唐静周义正李漂陈星星
Owner NINGBO AUCHEER BIOTECHNOLOGY CO LTD
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