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PEG/MoS2 quantum dot composite fluorescent nanometer microsphere and application thereof

A nano-microsphere and composite fluorescence technology, which is applied in the field of PEG/MoS2 quantum dot composite fluorescent nano-microspheres, achieves the effects of obvious pH responsiveness, simple process and short preparation period.

Inactive Publication Date: 2019-12-03
SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, MoS 2 The research of quantum dots mainly focuses on biological imaging, and its application in cancer treatment is still in the early stage of development, and MoS has not yet been seen. 2 Application of Quantum Dots in Fluorescence Imaging, Antitumor Drug Delivery and pH Responsive Drug Release Function as a Nano-Drug Carrier

Method used

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  • PEG/MoS2 quantum dot composite fluorescent nanometer microsphere and application thereof
  • PEG/MoS2 quantum dot composite fluorescent nanometer microsphere and application thereof
  • PEG/MoS2 quantum dot composite fluorescent nanometer microsphere and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1, PEG / MoS 2 Quantum dot composite fluorescent nanospheres (MoS 2 -PEG) preparation

[0062] Step 1. MoS 2 Preparation of quantum dots: 0.121g of Na 2 MoO 4 2H 2 O was dissolved in 12.5 mL of water, and after sonication for 5 min, the solution was adjusted to pH = 6.5 with 0.1 M HCl, then 0.615 g of glutathione (GSH) and 25 mL of water were added to the solution, sonicated for 10 min and argon was bubbled Finally, the mixture was transferred to a 50 mL Teflon-lined stainless steel autoclave and reacted at 200 °C for 24 h. Naturally cool to room temperature, centrifuge at 9000rpm for 5min to collect the supernatant, dialyze in pure water with a dialysis bag with a molecular weight cut-off of 500-1000 for 12-48h, and change the dialysate every 2-4h. Freeze-dry the dialyzed solution for 3 to 4 days to obtain MoS 2 quantum dots.

[0063] Step 2, MoS 2 -Preparation of PEG: 20mg of MoS 2 Quantum dots were dispersed in 5ml volume of PBS (0.1M, pH=7.4), MoS 2...

Embodiment 2

[0065] Example 2, PEG / MoS 2 Quantum dot composite fluorescent nanospheres (MoS 2 -PEG) preparation

[0066] Step 1. MoS 2 The preparation process of quantum dots is the same as that of Example 1.

[0067] Step 2, S1, 5mg of MoS 2 Quantum dots were dispersed in 5ml of PBS buffer (0.1M, pH=7.4) at a concentration of 1mg / mL, 2ml of 2.5% glutaraldehyde was added, then sonicated for 5min, and stirred at room temperature for 6h;

[0068] S2, the obtained suspension is dialyzed for the first time in pure water for 12 hours with a dialysis bag with a molecular weight cut-off of 1000, and the dialysate is changed every 4 hours to remove free glutaraldehyde;

[0069] S3. Add a PBS (0.1M, pH=7.4) solution (concentration: 1 mg / mL) of polyethylene glycol (PEG, average molecular weight 800) terminated by amino groups to the solution after dialysis, and the MoS in the resulting mixed solution 2 The mass ratio of quantum dots to PEG is 0.5:1, stirring for 8h;

[0070] S4. Carry out the ...

Embodiment 3

[0071] Example 3, PEG / MoS 2 Quantum dot composite fluorescent nanospheres (MoS 2 -PEG) preparation

[0072] Step 1. MoS 2 The preparation process of quantum dots is the same as that of Example 1.

[0073] Step 2, S1, 30mg of MoS 2 The quantum dots were dispersed in 5ml of PBS buffer solution (0.1M, pH=7.4) with a concentration of 6mg / mL, 10ml of glutaraldehyde with a concentration of 2.5% was added, then sonicated for 30min, and stirred at room temperature for 24h;

[0074] S2, the obtained suspension is dialyzed for the first time in pure water for 48 hours with a dialysis bag with a molecular weight cut-off of 500, and the dialysate is changed every 2 hours to remove free glutaraldehyde;

[0075] S3. Add a PBS (0.1M, pH=7.4) solution (concentration: 6 mg / mL) of double amino-terminated polyethylene glycol (PEG, average molecular weight 2500) to the solution after dialysis, and the MoS in the resulting mixed solution 2 The mass ratio of quantum dots to PEG is 4:1, stirrin...

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Abstract

The invention discloses a PEG / MoS2 quantum dot (MoS2-PEG) composite fluorescent nanometer microsphere and a preparation method and application thereof. The nanometer microsphere includes a PEG matrixand a MoS2 quantum dot embedded in the PEG matrix, the preparation method includes the steps of preparation of the MoS2 quantum dot and preparation of a nanometer microsphere MoS2-PEG, and according to the application, the MoS2-PEG is used as a carrier for loading anti-tumor drugs. According to the provided PEG / MoS2 quantum dot (MoS2-PEG) composite fluorescent nanometer microsphere, the EPR effectof tumor tissue is used for passively enriching the drugs into the tumor tissue, and the curative effect is improved while systemic toxicity is lowered; the preparation method is simple in process, mild in condition and short in preparation period, and harmful substances are not produced in the synthesis process; with the help of autofluorescence of the MoS2-PEG nanometer microsphere and the anti-tumor drugs, the synchronous tracer function is realized; drug release in vitro of the drug carrier system has obvious pH response, the drug loading capacity is high, and the PEG / MoS2 quantum dot (MoS2-PEG) composite fluorescent nanometer microsphere and the preparation method and application thereof can be widely used in the field of anti-tumor drug delivery.

Description

technical field [0001] The invention belongs to the field of nano-biomedicine and relates to a PEG / MoS 2 Quantum dot composite fluorescent nanosphere and its application. Background technique [0002] Cancer is a malignant tumor that seriously threatens human life and health. In 2018, there were 18.1 million new cancer cases and 9.6 million deaths worldwide. Cancer has become a global public health problem. As a populous country, China has the highest number of cancer deaths in the world. According to the "2018 China Cancer Report", an average of more than 10,000 people are diagnosed with cancer every day in my country, and 7 people are diagnosed with cancer every minute. How to cure cancer is a great challenge facing modern medicine. At present, the clinical treatment of tumors is still based on three methods: surgery, radiotherapy and chemotherapy, among which chemotherapy is the most commonly used method. However, traditional chemotherapy has problems such as serious...

Claims

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Application Information

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IPC IPC(8): C09K11/68A61K31/7048A61K9/51A61K47/10A61K47/02A61P35/00A61K49/00
CPCA61K9/5115A61K9/5146A61K31/7048A61K49/0019A61K49/0054A61K49/0067A61K49/0093A61P35/00C09K11/681
Inventor 董建刘莉董立峰
Owner SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
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