Needle electrode device matched with porous plate in use

Abstract

The invention provides a needle electrode device matched with a porous plate in use. The needle electrode device is characterized in that the needle electrode device comprises an electrode array and agasket, the gasket is provided with pores which are consistent with the electrode array in arrangement, the electrode array is fixed into the gasket and comprises a plurality of electrodes, and an electric pulse with first polarity and an electric pulse with second polarity are applied to the multiple electrodes according to the time period, wherein the electrodes corresponding to the electric pulse with the second polarity are distributed around the electrodes corresponding to the electric pulse with the first polarity. According to the needle electrode device, the upper part of the needle electrode array is sealed with the opened position of the porous plate, the problem of liquid sputtering during electrotransfection is prevented effectively, and cross contamination is avoided.

Description

technical field [0001] The invention relates to cell electrotransfection technology, in particular to a high-density distributed three-dimensional electrode device, in particular to an electrotransfection needle electrode device used in conjunction with a porous plate. Background technique [0002] Since the 1970s, electroporation technology has been used to insert molecules into animal cells or plant cells. Researchers have confirmed that exposure of cells to short-term and continuous high-voltage electric fields can form channels on the cell membrane, and macromolecules such as proteins and DNA can pass through. This channel enters the cell. These channels, known as electropores, are regions of increased permeability caused by local disruption of the cell membrane caused by high-voltage electric fields. These holes are short-lived but sufficient for the entry of large molecules such as plasmid DNA molecules into the cell. Cells can tolerate the formation of these holes, ...

AI Technical Summary

Problems solved by technology

[0007] Because there is a certain gap between the needle electrode array and the surface of the orifice plate, it is easier for the solution to splash out of the orifice plate during electrotransfection with the needle electrode array, which is not only a waste of the solution, but also may contaminate other orifice plates cells inside

[0008] In order to ensure that the needle-electrode array can completely touch the bottom, the actual length of the electrode needles should be slightly longer than the depth of the orifice plate. If the hand-held force is slightly larger during use, the electrode needles will easily bend and deform, which will cause a short circuit and generate electric shock sparks. even explode

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