An organic two-photon fluorescent probe, its preparation and application
A two-photon fluorescence and probe technology, which is applied in the field of biomedical imaging, can solve problems such as mining the optical properties of aggregate radiation, and achieve the effects of controllable probe size, high efficiency, and high temporal and spatial resolution
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Embodiment 1
[0050] Prepared with 100mg·mL -1 dimethyl sulfoxide solution of N-benzyloxycarbonyl-L-histidine-C-benzyl ester, add 985 μL 0.1 mg·mL to 10 μL of this solution -1 ICG aqueous solution or 0.1mg·mL -1 IR 806 aqueous solution, add 5 μL 100mM zinc chloride aqueous solution, oscillate to mix evenly, and age at 4°C in the dark for 24 hours to obtain ICG and IR 806 two-photon fluorescent probes respectively; the preparation contains 100mg·mL -1 The dimethyl sulfoxide solution of histidine derivatives, add 10 μL 10 mg·mL to 10 μL of the dimethyl sulfoxide solution -1 IR 806 dimethyl sulfoxide solution, add 975 μL of water to the mixed solution, add 5 μL of 100 mM zinc chloride aqueous solution, oscillate to mix evenly, and age at 4 ° C for 24 hours in the dark to obtain the IR 140 probe.
[0051] The obtained ICG, IR 140, and IR 806 two-photon fluorescent probes were photographed with an optical camera. The resulting probe is attached as figure 1 As shown, from left to right are IC...
Embodiment 2
[0053] Prepared with 200mg·mL -1 Chitosan solution, add 10μL 0.1M hydrochloric acid solution to promote dissolution, add 100μL of 1mg·mL -1 IR 806 aqueous solution, add 900 μL water to disperse, add 10 μL 0.1M sodium hydroxide solution to adjust the pH to 6-7.5 range. The prepared IR 806 two-photon two-photon fluorescent probe was aged at 4°C in the dark for 24 hours, and its particle size and potential were characterized by dynamic light scattering technology.
[0054] The particle size distribution figure of gained IR 806 two-photon fluorescent probe is as attached figure 2 , showing that the particle size is 113.1nm. The Zeta potential of the IR 806 two-photon fluorescent probe was measured to be -20.3mV.
Embodiment 3
[0056] Prepare 900μL containing 10mg·mL -1 Albumin aqueous solution, add 100μL 1mg·mL -1 The ICG aqueous solution was shaken to mix evenly, and aged at 4°C in the dark for 24 hours to obtain the ICG two-photon fluorescent probe.
[0057] The results obtained by scanning electron microscope characterization of the prepared ICG two-photon fluorescent probe are shown in the attached image 3 , the results showed that the prepared probes had uniform particle size and good dispersibility, and more than 90% of the particle sizes were within the range of the average particle size ±10%.
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