Ganoderma sp. CZ06 and application thereof
A strain, the technology of Ganoderma lucidum, applied in the field of microorganisms, can solve the problems of plastic foam fruit packaging, pollution, etc., and achieve the effect of loose and porous internal structure, good air permeability and good application prospects
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Embodiment 1
[0020] Example 1 Isolation, Purification and Preservation of Ganoderma Strain CZ06
[0021] The fruiting body of wild Ganoderma lucidum was collected from Anren Town, Dayi County, Chengdu City, Sichuan Province. The host plant was sycamore. The fruiting body of Ganoderma lucidum was collected and brought back to the laboratory for isolation.
[0022] Prepare PDA medium: 200g potatoes, 20g sucrose, 20g agar, natural pH, 1L distilled water.
[0023] The tested strains were isolated by tissue separation method. Under aseptic conditions, the surface of the collected Ganoderma lucidum is sterilized with sterile alcohol cotton, aseptically cut out the tissue pieces of the fruiting bodies of Ganoderma lucidum, and move them to PDA slant medium, culture at 25°C, observe the growth of mycelia, and pick out the contaminated tubes in time . According to the growth of the mycelia, the tip mycelium was picked and purified, combined with a microscope to obtain a pure culture, which was na...
Embodiment 2
[0025] ITS amplification and phylogenetic analysis of the strain CZ06 of embodiment 2
[0026] Extract the total DNA of the strain, use the total DNA as a template, use ITS4 (5'-TCCTCCGCT TATTGATATGC-3' SEQ ID No.2) and ITS5 (5'-GGAAGTAAAAGTCGTAACAAGG-3'SEQ ID No.3) as primers to amplify the ITS fragment, PCR reaction with Bio-RADMyCyclerTM instrument.
[0027] The reaction system (30 μl) is shown in Table 1: 15 μl of 2×Mix, 1 μl of primers ITS4 and ITS5, 1 μl of template DNA, and make up to 30 μl with ultrapure water.
[0028] PCR reaction conditions: pre-denaturation at 94°C for 5 min; denaturation at 94°C for 1 min, annealing at 54°C for 1 min, extension at 72°C for 2 min, 30 cycles; final extension at 72°C for 8 min.
[0029] The ITS amplified product was detected on 1.0% agarose gel electrophoresis, and sent to Shanghai Sangon Bioengineering Co., Ltd. for sequence determination. The sequence result is shown as SEQ ID No.1.
[0030] The obtained sequence results were co...
Embodiment 3
[0032] Example 3 Research on Growth Characteristics of Ganoderma Strain CZ06
[0033] (1) Determination of the growth temperature range of the strain: Inoculate CZ06 on the PDA solid medium, set 5 treatments of 15, 20, 25, 30, and 35°C, cultivate for 7 days, and observe the influence of different temperatures on the growth of mycelium. Record the average growth rate of mycelium and mycelial growth potential (Table 1).
[0034] Table 1 Effects of different temperatures on mycelium growth rate
[0035]
[0036] Note: +: mycelium is sparse, ++: mycelium is relatively dense, +++: mycelium is dense, ++++: extremely dense.
[0037] The results in Table 1 show that the growth temperature range of the tested strains is wide, ranging from 15°C to 35°C; the highest growth rate is 5.61±0.038mm d at 25°C -1, and its optimum growth temperature is 25°C.
[0038] (2) Determination of the pH range of the initial growth of the strain: adjust the pH of the PDA solid medium to 4, 6, 7, 8, ...
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