Bacillus megaterium, and microbial agent preparation and application thereof in energy saving and emission reduction

A technology of Bacillus megaterium and spores, which is applied in the preparation of Bacillus megaterium and its bacterial agent and its energy-saving and emission-reduction applications. It can solve the problems of unsuitable decentralized small-scale farmers, long repair period, secondary pollution, etc., and achieve excellent results. Environmental benefits and social benefits, reducing the amount of chemical fertilizers, reducing the effect of nitrogen loss

Inactive Publication Date: 2019-12-31
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, how to apply nitrogen fertilizer reasonably is a method that requires long-term research combined with effective field management, and is not suitable for most small-scale scattered farmers; and the application of nitrification inhibitors is likely to cause secondary pollution
The remediation methods of s

Method used

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  • Bacillus megaterium, and microbial agent preparation and application thereof in energy saving and emission reduction
  • Bacillus megaterium, and microbial agent preparation and application thereof in energy saving and emission reduction
  • Bacillus megaterium, and microbial agent preparation and application thereof in energy saving and emission reduction

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0022] Example 1

[0023] Screening of Bacillus megaterium NCT-5

[0024] This embodiment includes the following steps:

[0025] ①Cultivate the Bacillus megaterium CGMCC NO.4698 stored in the laboratory in a liquid medium (beef extract 3g / L, peptone 5g / L, NaCl 0.5g / L), 30℃, 200r / min in a shaker for 12h, Use a hemocytometer to determine the effective viable count concentration, and dilute to 10 8 CFU / mL bacterial suspension.

[0026] ②Using 30W ultraviolet lamp for strain mutation. Turn on the UV lamp before irradiation and preheat for 20 minutes. Use a pipette to draw 5 mL of bacterial suspension into a 9 cm sterile petri dish, adjust the distance between the UV lamp (30W) and the petri dish to 30 cm (vertical distance), open the lid of the dish, and process 30s, 60s, 90s, and 120s respectively. After mutagenesis, use a pipette to draw 0.2 mL of bacterial suspension to coat the plate (KNO 3 4g / L, MgSO 4 ·7H 2 O 0.5g / L, KH 2 PO 4 0.5g / L, KCl 1g / L, CaCl 2 1mg / L, FeSO 4 ·7H 2 O 10m...

Example Embodiment

[0034] Example 2

[0035] This example relates to the preparation of the Bacillus megaterium NCT-5 obtained in Example 1 into a solid inoculum, which specifically includes:

[0036] Seed medium: glucose 5g / L, beef extract 3g / L, peptone 5g / L, MgSO 4 ·7H 2 O 0.3g / L, adjust the pH to 6.5 with NaOH solution.

[0037] Spore production medium: bran 15g / L, potassium nitrate 10g / L, potassium dihydrogen phosphate 0.5g / L, magnesium sulfate 0.5g / L, manganese sulfate 0.1g / L, calcium carbonate 0.1g / L, using NaOH solution Adjust the pH to 7.0.

[0038] Bacillus megaterium (Bacillus megaterium) NCT-5 (CGMCC NO. 12910) was cultured for 10 hours under the condition of seed culture medium, 30℃, 200r / min shaking table, and the seed liquid was 2% (v / v) seed amount Inoculate in a 100L fermentor pre-filled with 60L of sterilized fermentation medium for culture. The culture conditions are 37°C, 200r / min, and aeration rate of 3.5L / min. The fermentation process does not require pH adjustment. After 12 hours...

Example Embodiment

[0039] Example 3

[0040] This embodiment relates to the preparation of Bacillus megaterium NCT-5 obtained in Example 1 into a liquid inoculum, which specifically includes:

[0041] Seed medium: glucose 5g / L, beef extract 3g / L, peptone 5g / L, MgSO 4 ·7H 2 O 0.3g / L, adjust the pH to 6.5 with NaOH solution.

[0042] Spore production medium: corn flour 10g / L, potassium nitrate 10g / L, soybean meal 5g / L, potassium dihydrogen phosphate 0.5g / L, magnesium sulfate 0.5g / L, manganese sulfate 0.1g / L, adjusted with NaOH solution pH 7.0.

[0043] Bacillus megaterium (Bacillus megaterium) NCT-5 (CGMCC NO. 12910) was cultured for 10 hours under the condition of seed culture medium, 30℃, 200r / min shaking table, and the seed liquid was 2% (v / v) seed amount It was inoculated in a 10L fermentor pre-filled with 7L of sterilized fermentation medium for culture. The culture conditions were 37°C, 300r / min, and aeration rate of 2.5L / min. The fermentation process does not require pH adjustment. Fermentation f...

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Abstract

The invention discloses bacillus megaterium as well as microbial agent preparation and application thereof, and specifically relates to Bacillus megaterium NCT-5CGMCC NO. 12910. Being jointly appliedwith a solid preparation, the bacterial strain liquid preparation disclosed by the invention is capable of effectively reducing nitrate content in soil and decreasing N2O emissions. The bacillus megaterium and the microbial agent preparation disclosed by the invention are easy to prepare, convenient to operate and low in processing cost, and can be widely used in the field of soil remediation.

Description

technical field [0001] The invention relates to a technology in the field of bioengineering, in particular to the preparation of bacillus megaterium and its bacterial agent and its application in energy saving and emission reduction. Background technique [0002] At present, domestic and foreign efforts to reduce farmland soil N 2 The main measure of O emission is rational application of nitrogen fertilizer and application of nitrification inhibitors. However, how to apply nitrogen fertilizer reasonably is a method that requires long-term research combined with effective field management, which is not suitable for most small-scale scattered farmers; and the application of nitrification inhibitors is likely to cause secondary pollution. The remediation methods of secondary salinized soil mainly include irrigation and salt washing, application of soil amendments, and planting of salt-tolerant plants, etc. Although these can also achieve the effect of soil remediation, they ar...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N13/00C12N1/36B09C1/10C12R1/11
CPCB09C1/10C12N1/20C12N1/36C12N13/00C12N1/205C12R2001/11
Inventor 张丹周培支月娥初少华蒋淼王斌戚志萍
Owner SHANGHAI JIAO TONG UNIV
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