Micromolecular composition for directionally inducing cell differentiation and preparation method of retinal pigment epithelial cells
A retinal pigment and cell differentiation technology, which is applied in the field of preparation of small molecule compositions and retinal pigment epithelial cells, can solve the problems of high cost, long differentiation cycle, and low differentiation efficiency, and achieve improved differentiation efficiency, low cost, and reliability Effect of culture preparation method
Active Publication Date: 2019-12-31
ZHENGZHOU UNIV
View PDF10 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
The small molecule composition can induce the directional differentiation of human embryonic stem cells into retinal pigment epithelial cells, in order to solve the technical problems of low induction differentiation efficiency, long differentiation cycle, and high cost in the prior art, so as to obtain high-purity retinal pigment epithelial cells for the treatment of Retinal Degenerative Disease Delivers Quality-Assured Therapeutic Cells
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment
[0036] Example: Culture method for inducing directed differentiation of human embryonic stem cells into retinal pigment epithelial cells
[0037] 1. Detection of Optimal Concentration of Niacinamide or Curcumin to Induce Retinal Pigment Epithelial Cells
[0038] (1) After hESC cell culture and subculture according to the routine hESC (human embryonic stem cell H1 cell line, commercially available) method, the cells are grown to a confluence state of 60-70%, and the boundaries between each clone are clear Before the contact is reached, the bFGF growth factor in the conventional culture medium used for hESC cell culture is removed, and the medium is changed once every 2 days. The cells reach complete fusion, and pigment foci are formed in about 3 to 4 weeks. Pigment foci were picked out with a glass electrode under a dissecting microscope, and moved to a culture dish covered with Matrigel. At the same time, the mTeSR1 medium was removed and replaced with retinal pigment epitheli...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
| Property | Measurement | Unit |
|---|---|---|
| concentration | aaaaa | aaaaa |
Login to View More
Abstract
The invention discloses a micromolecular composition for directionally inducing cell differentiation and a preparation method of retinal pigment epithelial cells, and aims to solve the technical problems of low induced differentiation efficiency, long differentiation period, high cost and the like. The micromolecular composition contains 5-50 ng / ml of nicotinamide, 0.5-10 [mu] M of curcumin, and the balance of water. The preparation method of the retinal pigment epithelial cells comprises the following steps: when fusing hESCs colonies to 60-70%, removing bFGF in a culture solution; culturinguntil pigment cells are formed, transferring the pigment cells to a culture dish paved with matrix glue, and replacing the culture dish with a retinal pigment epithelial cell culture medium; adding niacinamide on the first day to the tenth day of induced differentiation culture; starting from the eleventh day of the induced differentiation culture, adding the small molecule composition, and continuously culturing the material to the thirtieth day so as to obtain the product. According to the invention, high-purity retinal pigment epithelial cells can be obtained, and treatment cells with reliable quality are provided for treating retinal degenerative diseases.
Description
technical field [0001] The invention relates to the technical field of cell biology, in particular to a small molecule composition for directionally inducing cell differentiation and a preparation method of retinal pigment epithelial cells. Background technique [0002] my country is one of the countries with the largest number of blind people. The most common blinding diseases include retinal degeneration, glaucoma, cataract, corneal disease, etc., and the incidence of retinal degeneration is more than one-third of blinding eye diseases. There are more than 50 million patients with retinopathy in my country, and nearly 200,000 patients lose their sight due to such diseases every year. It is a serious blinding eye disease that has no effective treatment in the world. Retinal degenerative diseases mainly manifest as damaged degeneration or atrophy of the retinal pigment epithelium (RPE). PRE is a single layer of cells located between the retina and choroid, which plays a key...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071C12N5/079
CPCC12N5/0621C12N5/0625C12N2500/02C12N2500/30C12N2500/32C12N2500/44C12N2501/115C12N2502/02
Inventor 彭广华朱涵
Owner ZHENGZHOU UNIV