Application of olmesartan in preparation of medicines for delaying aging of vascular smooth muscle cells
A technology for vascular smooth muscle and cell aging, which is applied in drug combinations, cardiovascular system diseases, and pharmaceutical formulations to achieve the effect of delaying the aging of vascular smooth muscle cells
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[0025] In order to make the technical problems, technical solutions and advantages to be solved by the present invention clearer, the following will describe in detail in conjunction with specific embodiments.
[0026] 1. Olmesartan delays the aging of vascular smooth muscle cells
[0027] Experimental group:
[0028] ① Control group: HA-VSMCs (human vascular smooth muscle cells) without intervention;
[0029] ②Bleomycin-induced aging group: HA-VSMCs were treated with bleomycin for 48 hours;
[0030] ③Olmesartan intervention group: HA-VSMCs were pretreated with olmesartan for 2 hours and then combined with bleomycin for 48 hours.
[0031] The above three experimental groups were used to construct the cellular aging model. Experimental method: staining with SA-β-Gal, NAD + / NADH ratio measurement, γ-H2AX fluorescence staining to detect DNA damage, and EdU flow cytometry to quantitatively measure cell proliferation ability indicators to evaluate cell senescence.
[0032] Th...
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