InDel molecular marker 5mBi3 for identifying bitterness traits of melon fruit and primer and application thereof

A technology of molecular markers and melons, which is applied in the field of molecular biology, can solve the problems of less work in molecular marker selection and breeding, and achieve the effects of improving accuracy and selection efficiency, high accuracy, and convenient detection

Active Publication Date: 2020-01-03
ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
View PDF9 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, in melon varieties, there are relatively few genetic studies on bitterness genes and related molecular marker selection.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • InDel molecular marker 5mBi3 for identifying bitterness traits of melon fruit and primer and application thereof
  • InDel molecular marker 5mBi3 for identifying bitterness traits of melon fruit and primer and application thereof
  • InDel molecular marker 5mBi3 for identifying bitterness traits of melon fruit and primer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Analysis of genetic characteristics of muskmelon fruit bitterness and material screening

[0027] C68 (female parent) and C69 (male parent) (C68 is Primo X Fengtian No. 1 selection line, C69 is Xiao Cuigua X Guangzhou Mi selection line) selected from the West Melon Medium-term Bank of Zhengzhou Institute of Fruit Trees, Chinese Academy of Agricultural Sciences as parents , crossed to obtain F 1 , F 1 F 2 , F 1 96 strains of BC were obtained by backcrossing with the female parent 1 group.

[0028] Among them, C69 melon fruit was identified as bitter, C68 fruit was identified as not bitter, F 1 The fruits of muskmelon were identified as bitter, indicating that bitterness is dominant relative to non-bitterness.

[0029] In 96 F 2 In the generation population, there were 92 plants with bitter young fruit and 4 plants with no bitterness. The Chi-square test showed that the segregation ratio of Mendelian inheritance was 15:1 (χ 2 =0.71, p -value=0.40); in ...

Embodiment 2

[0031] Example 2: Mapping of genes related to bitterness traits of melon fruit and development of InDel molecular markers

[0032] (1) Preliminary positioning of the main QTL intervals for bitterness traits in melon fruits

[0033] Parental and BC analysis by 2b-RAD sequencing method 1 The population was sequenced to excavate relevant SNP sites, and the genetic linkage map of muskmelon was constructed using JoinMap4.0 software, and the 95 BC strains 1 The group tasted fruit bitterness; combined with the results of genetic linkage map and bitter taste taste, the MapQTL6.0 software was used for initial QTL mapping, as shown in figure 1 As shown, a main effect QTL interval related to the bitterness trait of melon fruit was detected on LG3. LG3 corresponds to chromosome 2, the LOD peak value is 6.65, which can explain 27.6% of the phenotypic variation, the confidence interval is 54.335-74.628cM, and the corresponding physical location is 17238358bp-24543640bp; Effective QTL in...

Embodiment 3

[0037] Example 3: Indel marker analysis verification test of melon X92 population

[0038] (1) Test material

[0039] The C68 (female parent), C69 (paternal parent) and 70 X92 populations in Example 1 were used as test materials.

[0040] (2) Genotype identification of X92 population

[0041] With C68 (female parent), C69 (father parent) and F 1A single plant was used as the experimental material, and polymorphism screening was carried out using the InDel primers designed in Example 2, and then genotype identification was carried out in the X92 population using the polymorphic InDel primers, and finally the 21653588bp located on chromosome 5 was obtained. The bitterness trait of melon is closely related to the Indel marker, named 5mBi3, its nucleotide sequence is 5'-TGTGTGTGTGTGTGTG-3', and its PCR primers are shown in Table 1 below.

[0042] Table 1 Specific primer information

[0043] .

[0044] The specific steps of screening are as follows:

[0045] (1) Extraction ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an InDel molecular marker 5mBi3 for identifying bitterness traits of melon fruit and a primer and application thereof. The technical problem that the germplasm identification of the bitterness traits of the melon fruit cannot be quickly and accurately performed in the prior art is solved. The InDel molecular marker for identifying the bitterness traits of the melon fruit isselected, the primer for the InDel molecular marker is designed, a method for identifying the bitterness traits of the melon fruit is provided, and the molecular marker, the primer or the method is applied to molecular marker assisted breeding of the bitterness traits in the melon fruit. The molecular marker can accurately identify whether the melon fruit contains bitterness at the seedling stageor not, detection is convenient, the accuracy is high, no influence of environmental factors exists, a new method to identify the bitter taste of the melon fruit can be provided, the improvement of bitter melon varieties is accelerated, and the breeding accuracy and selection efficiency are improved.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to an InDel molecular marker 5mBi3 for identifying bitterness traits of melon fruits, primers and applications thereof. Background technique [0002] melon( Cucumis melo L.) is an important dicotyledonous plant of the Cucurbitaceae family with a wide distribution worldwide. The bitterness of young muskmelon fruit is an important quality trait, and the difference between bitterness and non-bitterness is obvious, which seriously affects the quality of muskmelon fruit. Studying the bitterness of young muskmelon fruit is of great value for genetic breeding and germplasm origin research. [0003] With the development of high-throughput sequencing and molecular marker technology, whole genome resequencing and analysis of sequence information, molecular marker-assisted breeding technology has become one of the most widely used technologies in crop genetics and breeding. The c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 周丹马双武王吉明尚建立李娜孔胜楠李楠楠
Owner ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap