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Sample pretreatment method suitable for immunological detection of drug residues in food

An immunological detection and sample pretreatment technology, applied in the field of sample pretreatment for immunological detection of drug residues in food, can solve problems such as affecting the accuracy of immunological detection methods and reducing the detection efficiency of target objects, and achieves convenient operation and extraction. The effect of improved efficiency and simple extraction method

Inactive Publication Date: 2020-01-10
MEIZHENG BIO TECH CO LTD +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the existing sample pretreatment method, the extracted target substance is mostly dispersed in the organic solvent, but the organic solvent affects the accuracy of the immunological detection method, so it is necessary to increase the step of removing the organic solvent before performing the immunological detection. Thereby reducing the detection efficiency of the target

Method used

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  • Sample pretreatment method suitable for immunological detection of drug residues in food
  • Sample pretreatment method suitable for immunological detection of drug residues in food
  • Sample pretreatment method suitable for immunological detection of drug residues in food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Detection of nitrofuran metabolites

[0023] 1. Sample pretreatment

[0024] Weigh 6g of homogeneous tissue sample into a 50mL centrifuge tube, add 4mL of deionized water, 5mL of 1mol / L hydrochloric acid (3.1.14) and 0.2mL of 10mmol / L o-nitrobenzaldehyde solution in sequence, shake fully for 3min; Incubate the tube in a 60°C water bath for 60min; add 5mL 0.1mol / L dipotassium hydrogen phosphate solution, 0.4mL 1mol / L sodium hydroxide solution, and 6mL ethyl acetate in sequence, mix thoroughly for 3min, and incubate at room temperature (20-25°C). Centrifuge at 4000r / min for 5min; pipette 3mL of the supernatant liquid after centrifugation into a 15mL centrifuge tube, add 10mL of n-hexane solution, mix up and down 4-5 times, and centrifuge at 4000r / min for 1min. Connect the negative pressure solid phase extraction device, and connect a 30mL syringe syringe above the solid phase extraction column, pour all the above supernatant into the 30mL syringe, turn on the a...

Embodiment 2

[0028] Example 2 Chloramphenicol Detection

[0029] 1. Sample pretreatment

[0030] Weigh 5g of animal tissue samples and homogenize them in a homogenizer, place the homogenized tissue samples in a centrifuge tube; then add 5mL of 20% sodium chloride aqueous solution to shake and mix well, then add 10mL of ethyl acetate extractant to fully Mix for 2-5min, centrifuge at 4000r / min for 10min at room temperature at 20-25°C, absorb 5mL of centrifuged supernatant, add 15mL of petroleum ether, shake for 1min to mix evenly, then add 1mL of 0.01M PBS , shake for 1 min to make it evenly mixed, centrifuge or stand still, and collect the layered lower aqueous phase. If necessary, use 1~5mL n-hexane or petroleum ether to extract the organic solvent in the lower liquid again.

[0031] 2. Add recycling experiment

[0032] Different contents of chloramphenicol were added to the negative samples, and the contents of chloramphenicol were 0 (blank control), 0.05, 0.1, 0.2 μg / kg. The negative...

Embodiment 3

[0034] Example 3 Metronidazole detection

[0035] 1. Sample pretreatment

[0036] Weigh 5g of animal tissue samples and homogenize them in a homogenizer, place the homogenized tissue samples in a centrifuge tube; then add 10mL ethyl acetate to shake and extract for 3min, and centrifuge at room temperature (20-25°C) at 4000r / min for 5min ; Pipette 5 mL of the centrifuged upper layer liquid; then add 10 mL of n-hexane and 5 mL of petroleum ether, shake for 2 minutes to make it evenly mixed, connect the negative pressure solid phase extraction device, and connect a 30 mL syringe barrel above the solid phase extraction column. Pour the mixed solution into a 30mL syringe barrel, turn on the negative pressure suction device, let all the liquid flow through the solid phase extraction column, control the liquid flow rate to about 1 drop / second, remove the solution in the solid phase extraction column as much as possible, and then close the negative pressure extraction device. Pressur...

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Abstract

The invention provides a sample pretreatment method suitable for the immunological detection of drug residues in food. The method comprises the steps of (1) treating a homogeneous tissue sample with an extracting solution and centrifuging the treated sample to collect supernate, (2) mixing the supernate collected in the step (1) with a polarity regulator to obtain a mixed solution, wherein the mixed solution and an aqueous solution are immiscible, and (3) transferring drug residues in the mixed solution obtained in the step (2) into the aqueous solution or a buffer solution with the pH value of 5.0-9.0 and directly applying the collected aqueous solution or buffer solution with the pH value of 5.0-9.0 to immunological detection and analysis. A target object extracted by the method is freeof organic solvent interference and can be directly used for immunological method detection, operation steps are simplified, the extraction efficiency and the detection efficiency are improved, and meanwhile, an immunodetection result is not influenced, and so that the method has important application value in pretreatment application of the drug residues in the food.

Description

technical field [0001] The invention belongs to the field of food safety detection, and in particular relates to a sample pretreatment method suitable for immunological detection of drug residues in food. Background technique [0002] With the advancement of science and technology and the improvement of people's living standards, the requirements for food safety are becoming increasingly stringent, so the research on the effectiveness of food safety detection technology is more urgent. To achieve this goal, in addition to raising people's awareness of prevention, we must choose a convenient and popular detection method for supervision. The development of immunological detection technology provides a technical possibility for the realization of this goal. [0003] Immunological methods are established on the basis of specific reactions of antigens and antibodies, and there are many kinds of detection methods derived from them. Almost all immunological methods can be used for ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/34
CPCG01N1/34
Inventor 张勋田秀梅孙成
Owner MEIZHENG BIO TECH CO LTD