Method for producing bostrycin by solid-state fermentation of HCH285 strain

A solid-state fermentation and strain technology, applied in the field of fermentation, can solve the problems of complex process, low extraction rate and high cost, and achieve the effect of short fermentation time and high product content

Inactive Publication Date: 2020-01-31
湖北省荆楚药材研究院
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  • Abstract
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  • Application Information

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Problems solved by technology

The extraction of natural anti-tumor drugs from plant and animal raw materials is restricted by many conditions such as season, climate, and

Method used

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  • Method for producing bostrycin by solid-state fermentation of HCH285 strain
  • Method for producing bostrycin by solid-state fermentation of HCH285 strain
  • Method for producing bostrycin by solid-state fermentation of HCH285 strain

Examples

Experimental program
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Example Embodiment

[0021] Example 1

[0022] 1. The method for producing bostrycin by solid-state fermentation of HCH285 using rice fermentation medium includes the following steps:

[0023] Step 1. Strain activation

[0024] Inoculate the wormwood endophytic fungus HCH285 on PSA plate medium (cut the washed and peeled potatoes, add 1000mL of water and boil for about half an hour, filter out the potatoes with 4 layers of gauze, then add 20g of sucrose and 15g of agar, add water Make up to 1000mL, stir to dissolve, and distribute into Erlenmeyer flasks, sterilize under high pressure steam at 121℃ for 20min, and distribute into petri dishes before activation.) Cultivate at 22~26℃ until the colonies are dense and the colonies are red.

[0025] Step 2. Preparation of first-level seed liquid

[0026] Use a hole puncher with a hole diameter of 0.9cm to punch 2 holes of mycelium on the activated plate seeds, and then inoculate them in PSB liquid medium (the washed and peeled potatoes are chopped, add 1000mL of ...

Example

[0032] Example 2-Example 6

[0033] In Example 2 to Example 6, 20g of rice was weighed into an Erlenmeyer flask. The difference was that the ratio of the volume of the initial water added to the rice mass was 40%, 50%, 70%, 80%, 90%, respectively. , Other culture conditions are the same as in Example 1.

Example

[0034] Example 7-Example 12

[0035] The initial pH in Example 7 to Example 12 were 3, 4, 5, 6, 7, and 8, respectively, and other culture conditions were the same as in Example 1.

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Abstract

The invention provides a method for producing bostrycin by using solid-state fermentation of an HCH285 strain. The method inoculates a secondary seed liquid of the Qiai endophytic fungus HCH285 in a solid fermentation substrate for cultivation, wherein the solid fermentation substrate is rice or mashed potato. The method increases the content of the bostrycin in the fermentation product, and has the characteristics of a short production cycle, a high yield and convenient operation.

Description

technical field [0001] The invention relates to the technical field of fermentation, in particular to a method for producing bostrycin by solid-state fermentation of HCH285 strain. Background technique [0002] Malignant tumor, commonly known as cancer. The results of the third national cause of death survey show that the death rate caused by malignant tumors ranks second among all causes of death among urban residents in my country, accounting for 22.32% of the total death rate, and both the morbidity rate and the death rate show a continuous upward trend. In the past 20 years, the proportion of malignant tumor deaths among urban residents in my country has increased from 21.88% to 26.33%. Malignant tumor has become a major disease that seriously threatens human life and health, and it shows a continuous growth trend. Although there are a large number of anticancer drugs, most of them in clinical practice have problems such as strong cytotoxicity, poor selectivity, and hi...

Claims

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Application Information

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IPC IPC(8): C12P15/00C12N1/14C12R1/645
CPCC12N1/14C12P15/00
Inventor 王沫揭晶石乐王璋倩熊涛
Owner 湖北省荆楚药材研究院
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