Method for rapidly detecting content of vitamin C in vegetables and fruits
A vitamin and vegetable technology, which is applied to measurement devices, analyzes materials by chemical reaction, and analyzes materials by observing the effect on chemical indicators, can solve the problem of high cost of replacing sensors, easy contamination of sensors, and measurement accuracy. Low problems, to achieve the effect of convenient operation, reduce measurement costs, and improve accuracy
Pending Publication Date: 2020-02-07
HUAIBEI NORMAL UNIVERSITY
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Problems solved by technology
This method is simple and fast, but the measurement accuracy is low. Spectrophotometry, fluorescence and high performance liquid chromatography have the advantages of high sensitivity, good selectivity, rapid determination, and not affected by color.
However, the required equipment is expensive, bulky, and requires professionals to operate
The electrochemical method is simple and easy, but the sensor is easily contaminated, and the cost of replacing the sensor is high
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Embodiment 1
[0033] A method for quickly detecting vitamin C content in vegetables and fruits, comprising the steps of:
[0034] S1, add 0.6mL of 4.0mg / mL o-phenanthroline solution and 0.6mL of 2.4mg / mL ferric ammonium sulfate solution in turn to 8.8mL vitamin C standard solution with a concentration of 0-20μg / mL, and finally add sodium acetate buffer solution, adjust the pH to 4-5, carry out the color reaction, and use the same dosage and concentration of phenanthroline solution and ferric ammonium sulfate solution to prepare a blank solution.
[0035] S2, collecting the color image of the color-developed vitamin C standard solution, and obtaining the Lab value of the color image, the specific steps are as follows:
[0036] Step 1, extracting the RGB values of 400 pixels in the central region of the color image;
[0037] Step 2, calculate the RGB mean value;
[0038] Step 3, convert the RGB mean value into an XYZ value through color space conversion, and then convert the XYZ value int...
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Abstract
The invention relates to a method for detecting the content of vitamin C, in particular to a method for rapidly detecting the content of vitamin C in vegetables and fruits. According to the method, Fe(III) is quantitatively reduced into Fe(II) by utilizing vitamin C, and Fe(II) reacts with phenanthroline to generate a stable orange red complex. The method comprises the following steps of: acquiring an image of a standard vitamin C solution with a known concentration by using a camera, processing the image to obtain a corresponding RGB value, converting the RGB value into an XYZ value, and converting the XYZ value into a Lab value; carrying out multivariate data fitting on the Lab value and the concentration of the vitamin C standard solution, and establishing the standard curve and the multivariate fitting expression of the concentration and the color component of the vitamin C standard solution; when the content of vitamin C in vegetables and fruits is actually determined, obtaining aconcentration detection value only by substituting the Lab value of the developed image of the fruit and vegetable extracting solution into the established multivariate fitting expression, and calculating the content of vitamin C in fruits according to the concentration. The method is simple in design and convenient to operate, and can replace a traditional spectrophotometer to determine the content of vitamin C in fruits and vegetables.
Description
technical field [0001] The invention relates to a method for detecting vitamin C content, in particular to a method for rapidly detecting vitamin C content in vegetables and fruits. Background technique [0002] Vitamin C, also known as ascorbic acid, is a water-soluble vitamin with strong antioxidant properties. Vitamin C optimizes cellular and tissue levels to prevent and treat respiratory and systemic infections. High doses of vitamin C can be used as a cheap, efficient and multifaceted antioxidant, which can prevent or restore microcirculatory disturbance, and at the same time have a protective effect on excessive oxidative stress caused by ischemia, sepsis or burns, reduce organ damage and disfunction. Vitamin C deficiency can lead to impaired immunity and higher susceptibility to infection. Most mammals can synthesize vitamin C from glucose in the liver, but humans, non-human primates and guinea pigs cannot synthesize vitamin C themselves. When the intake of vitamin...
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IPC IPC(8): G01N21/78G01N21/84
CPCG01N21/78G01N21/8483
Inventor 刘升赵文竹许海杰肖建于葛方振陈德宝宋万干
Owner HUAIBEI NORMAL UNIVERSITY