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Method for detecting content of SA alpha 2-3Gal sugar chain in saliva sample by ELISA

A technology of sugar chains and samples, which is applied in the field of quantitative detection of sialic acid sugar chains, can solve the problems of high experimental cost, unseen SAα2-3Gal sugar chains, cumbersome experimental steps, etc., and achieve accurate quantitative results

Active Publication Date: 2020-02-07
陕西医药控股医药研究院有限公司
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Problems solved by technology

For example, the ELISA-based detection method has no suitable standard, so this method can only carry out relative quantification of SAα2-3Gal sugar chain content, but cannot perform absolute quantification; while using high performance liquid chromatography to quantitatively detect SAα2-3Gal sugar chain, It is necessary to specifically hydrolyze the target sugar chain into a free state, and use the sugar chain of the same structure as a standard for quantitative detection, so the experimental steps are cumbersome and the experimental cost is high
At present, there is no method to quantitatively detect SAα2-3Gal sugar chains in samples using fetuin as a standard

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  • Method for detecting content of SA alpha 2-3Gal sugar chain in saliva sample by ELISA
  • Method for detecting content of SA alpha 2-3Gal sugar chain in saliva sample by ELISA
  • Method for detecting content of SA alpha 2-3Gal sugar chain in saliva sample by ELISA

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Embodiment Construction

[0029] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments.

[0030] 1. Detection principle

[0031] see figure 1 , the present invention is based on ELISA technology, that is, using the specific binding reaction between lectin and the sugar chain on the glycoprotein (Glycoprotein), the saliva sample to be tested (the detection target is the SAα2-3Gal sugar chain) and the standard are fixed on a solid After phase carrier surface, directly use biotin (Biotin)-labeled specific lectin to incubate with the saliva sample to be tested and the standard respectively (that is, add biotinylated lectin that specifically recognizes the target sugar chain structure, so that the glycoprotein surface is ready to To detect the combination of sugar chains and the lectin, the lectin used in the present invention that can specifically recognize SAα2-3Gal sugar chains is MAL-II), and then labeled with horseradish peroxidas...

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Abstract

The invention discloses a method for detecting the content of an SA alpha 2-3Gal sugar chain in a saliva sample by ELISA. According to the method, a fetal globulin is used as a standard substance, andthe content of the fetal globulin which has the same content as the SA alpha 2-3Gal sugar chain in the saliva sample under a certain saliva protein coating concentration can be calculated by utilizing a standard curve, so that the content of the SA alpha 2-3Gal sugar chain in the saliva sample can be calculated, and the quantitative result is accurate.

Description

technical field [0001] The invention relates to the quantitative detection of sialic acid sugar chains, in particular to a method for detecting the content of SAα2-3Gal sugar chains. Background technique [0002] Enzyme-linked immunosorbent assay (ELISA) is an enzyme-linked immunoassay technique. It uses the specific combination of antigen and antibody to connect the analyte to the enzyme. After adding the substrate of the enzyme reaction, the substrate is catalyzed by the enzyme to become a colored substance, and an absorbance value is generated at a certain wavelength. The absorbance value of the product is the same as If the amount of the analyte in the sample is directly related, the content of the analyte in the sample can be quantitatively detected according to the absorbance value. [0003] Lectin is a kind of protein or glycoprotein extracted from various plants, invertebrates and higher animals, which can recognize a specific sugar chain structure (can be combined ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/58G01N33/543
CPCG01N33/68G01N33/581G01N33/543G01N2400/02
Inventor 李铮王喜龙秦湫红张志伟麻纪斌任建平
Owner 陕西医药控股医药研究院有限公司
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