Method for detecting content of SA alpha 2-3Gal sugar chain in saliva sample by ELISA

A technology of sugar chains and samples, which is applied in the field of quantitative detection of sialic acid sugar chains, can solve the problems of high experimental cost, unseen SAα2-3Gal sugar chains, cumbersome experimental steps, etc., and achieve accurate quantitative results
CN110763849AActive Publication Date: 2020-02-07陕西医药控股医药研究院有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
陕西医药控股医药研究院有限公司
Publication Date
2020-02-07

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Abstract

The invention discloses a method for detecting the content of an SA alpha 2-3Gal sugar chain in a saliva sample by ELISA. According to the method, a fetal globulin is used as a standard substance, andthe content of the fetal globulin which has the same content as the SA alpha 2-3Gal sugar chain in the saliva sample under a certain saliva protein coating concentration can be calculated by utilizing a standard curve, so that the content of the SA alpha 2-3Gal sugar chain in the saliva sample can be calculated, and the quantitative result is accurate.
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Description

technical field

[0001] The invention relates to the quantitative detection of sialic acid sugar chains, in particular to a method for detecting the content of SAα2-3Gal sugar chains. Background technique

[0002] Enzyme-linked immunosorbent assay (ELISA) is an enzyme-linked immunoassay technique. It uses the specific combination of antigen and antibody to connect the analyte to the enzyme. After adding the substrate of the enzyme reaction, the substrate is catalyzed by the enzyme to become a colored substance, and an absorbance value is generated at a certain wavelength. The absorbance value of the product is the same as If the amount of the analyte in the sample is directly related, the content of the analyte in the sample can be quantitatively detected according to the absorbance value.

[0003] Lectin is a kind of protein or glycoprotein extracted from various plants, invertebrates and higher animals, which can recognize a specific sugar chain structure (can be combined ...

Claims

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