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Tobacco fusarium root rot inhibitor

A technology for fusarium and root rot, which is applied in the directions of fungicides, biocides, biocides, etc., can solve the problems of difficult control, difficult control, and harm of tobacco root rot, and achieves inhibition of spore germination and mycelial growth. , the prevention effect is stable, the effect of reducing environmental pollution

Inactive Publication Date: 2020-02-11
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the control method of tobacco fusarium root rot mainly adopts chemical control. Due to the lack of specific agents and resistant varieties, the control of tobacco root rot is difficult and the damage is serious. It has become one of the most difficult and important diseases in production. (Zhao Jie et al., 2013)

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0018] Example 1 Inhibitory effect of allelochemical substances on mycelia growth of Fusarium oxysporum root rot

[0019] Refer to the method of Zhou Baoli et al. (2011): four kinds of garlic root exudates allelochemicals dibutyl phthalate, 2,6-diisopropylphenol, diallyl disulfide, 2,6-ditertiary Butyl-p-cresol uses methanol as a solvent to prepare garlic root exudate allelochemical reagents. Add four kinds of garlic root exudate allelochemical reagents to the PDA medium so that the respective concentrations in the medium are 0.05, 0.1, 0.5, 1 and 5mmol / L, with the addition of an equal amount of methanol as a control (CK). Gently rotate the Erlenmeyer flask to mix the garlic root exudate allelochemical reagent with the medium evenly, pour the mixture into a 9cm-diameter petri dish, 20mL per dish, and after cooling and solidifying, insert one Tobacco Fusarium oxysporum root rot bacterial cake with a diameter of 5 mm was cultured in the dark at 28°C. Three repetitions were set...

Embodiment 2

[0026] Example 2 Determination of the influence of allelochemicals from garlic root exudates on spore germination of root rot (Fusarium oxysporum)

[0027] Refer to Zhou Yanli’s (2011) method: use a puncher to punch a 0.5 cm diameter bacterial cake along the edge of the colony that has grown for 4 days, and inoculate the tobacco root rot fungus on PDA oat medium for 7 days at 28 °C in the dark during sporulation Scrape the mycelia until the mycelium is luxuriant, put the mycelium in sterile water, wash the spores with sterile water, shake well, check under the microscope, and adjust the concentration of spores to 1×10 7 cfu / mL. Dilute dibutyl phthalate, 2,6-diisopropylphenol, diallyl disulfide, and 2,6-di-tert-butyl-p-cresol from garlic root exudates with 20% methanol to 0.05, 0.1, 0.5, 1 and 5mmol / L, with 20% methanol as the control. Draw 100 μL of bacterial suspension, mix with 100 μL of garlic root exudate allelochemical reagents of different concentrations to form a mixe...

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Abstract

The invention provides a tobacco fusarium root rot inhibitor, and belongs to the field of plant protection. The inhibitor includes 0.05-5 mmol / L of 2,6-diisopropylphenol. The inhibitor can effectivelyinhibit the growth of tobacco fusarium, and when the concentration is 5 mmol / L, the inhibition rate of the inhibitor on the growth of mycelia of the tobacco fusarium root rot is 83.46%, and the sporegermination inhibition rate is 100%. The effective active ingredient of the inhibitor is 2,6-diisopropylphenol which is a botanical active substance garlic root exudate, so that the control effect isstable; and the inhibitor has good effects of inhibiting spore germination and hypha growth of the bacteria, can reduce the use of chemical pesticides and reduce environmental pollution, and also canimprove the quality of tobacco leaves.

Description

technical field [0001] The invention belongs to the field of plant protection, in particular to an inhibitor of tobacco fusarium root rot. Background technique [0002] Tobacco Fusarium root rot is one of the main rhizome diseases, and tobacco root rot occurs commonly in Fujian Province. The pathogenic bacteria are Fusarium oxysporum ( F. oxysporum) , Fusarium solani ( F. solani ) and half-naked Fusarium ( F. Semitectum ) (Wu Anzhong et al., 2018; Zhang Shaosheng et al., 2012). Due to the global warming, the accumulation of pathogenic bacteria caused by continuous cropping, the perennial application of chemical fertilizers and pesticides in tobacco fields, and the reduction of organic fertilizer application, the soil microbial community is unbalanced, and tobacco root rot is increasing year by year (Zhu Xianchao et al., 2001; Cole J D , et al., 1982). At present, the control method of tobacco fusarium root rot mainly adopts chemical control. Due to the lack of specif...

Claims

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Application Information

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IPC IPC(8): A01N31/08A01P3/00
CPCA01N31/08
Inventor 吴晓婷赖荣泉王巧妮
Owner FUJIAN AGRI & FORESTRY UNIV
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