Method for determining pesticide residue in vegetables by gas chromatography

Abstract

The invention discloses a method for determining pesticide residues in vegetables by gas chromatography. The method includes the following steps: pretreating a sample, taking 15.00 g of a homogeneoussample to be tested into a 200 mL flask, adding 15 mL of acetonitrile, homogenizing the sample with a high-speed homogenizer for 90 seconds, filtering the sample into a stoppered cylinder containing 5g of sodium chloride in advance, shaking the cylinder well, and standing for 25min; transferring 15mL of the supernatant to a small beaker, and adding about 8g of anhydrous sodium sulfate to well mix;purifying, whirling in a vortex mixer for 10min, centrifuging at 4000r / min for 5min, collecting the acetonitrile solution into a concentration bottle, adding 5mL of acetonitrile and repeating the extraction, making up the volume to 2mL with acetone, purifying with a ZuEChERS purification tube; carrying out gas phase detection by the gas chromatography; and checking the recovery rate, and analyzing the results. In the present invention, the extraction solution is appropriately concentrated and then subjected to purification treatment to improve the detection sensitivity of the method. In this method, consumption of solvents is reduced and time is saved. The method is suitable for the rapid screening of pesticide residues in vegetable samples. The method enables high detection efficiencyand is worth popularizing and applying.

Description

technical field [0001] The invention belongs to the detection field, and in particular relates to a method for determining pesticide residues in vegetables by gas chromatography. Background technique [0002] The development of agricultural industrialization makes the production of agricultural products more and more dependent on exogenous substances such as pesticides, antibiotics and hormones. The amount of pesticides used in agricultural products in my country remains high, and the unreasonable use of these substances will inevitably lead to excessive pesticide residues in agricultural products, affecting the safety of consumers, and in severe cases, it will cause consumers to become ill, develop abnormally, and even directly lead to poisoning die. Excessive pesticide residues will also affect the trade of agricultural products. Countries all over the world attach great importance to the issue of pesticide residues, and have set increasingly strict limits for pesticide re...

AI Technical Summary

Problems solved by technology

The amount of pesticides used in agricultural products in my country remains high, and the unreasonable use of these substances will inevitably lead to excessive pesticide residues in agricultural products, affecting the safety of consumers, and in severe cases, it will cause consumers to become ill, develop abnormally, and even directly lead to poisoning die

Images