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A bacterial strain capable of producing DHA by high-temperature fermentation and its application

A technology of high-temperature fermentation and bacterial strains, which is applied in the direction of fermentation, microbial-based methods, fungi, etc., can solve the problems of limited application range of DHA oil commercialization, high fermentation production cost, and reduced DHA production, and achieves cell growth. The effect of shortening the fermentation period and increasing the growth rate

Active Publication Date: 2021-08-03
NANJING TECH UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] However, in the existing methods, the suitable temperature for the fermentation of Schizochytrium to produce DHA is generally 25-30 degrees. If the fermentation temperature exceeds 30° C., the growth of Schizochytrium will be inhibited, the DHA output will be significantly reduced, and its fermentation production cost is high. The market is expensive, so the commercial application of DHA oil produced by microorganisms is still limited

Method used

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  • A bacterial strain capable of producing DHA by high-temperature fermentation and its application
  • A bacterial strain capable of producing DHA by high-temperature fermentation and its application
  • A bacterial strain capable of producing DHA by high-temperature fermentation and its application

Examples

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Embodiment 1

[0022] This example specifically illustrates the screening method for the strains of the present invention.

[0023] (1) Activating the original Schizochytrium strain at 30° C.; activating the original strain for 3 generations, each generation time is 24-48 hours.

[0024] (2) Insert the activated seeds into the seed medium with an inoculation amount of 1% (v / v), and the culture temperature is 32°C, 34°C, 34.5°C and 35°C, and the temperature can be raised according to the growth of the strain For acclimatization, for example, when the strain grows well at 32°C, the acclimatization temperature of the next generation can be set to 34°C, and so on, the rotation speed is 170rpm, and the shaking table is cultivated until the logarithmic growth phase of the strain is transferred to the next generation;

[0025] The composition of the seed medium is: glucose 30-60g / L, yeast powder 3-5g / L, MgSO 4 ·7H 2 O 4~6g / L, Na 2 SO 4 3~5g / L, (NH 4 ) 2 SO 4 4~6g / L,KH 2 PO 4 2~4g / L.

...

Embodiment 2

[0029] This example specifically illustrates the method for producing DHA by fermentation of Schizochytrium in the present invention.

[0030] (1) Seed cultivation

[0031] The composition of the seed medium is: glucose 30-60g / L, yeast powder 3-5g / L, MgSO 4 ·7H 2 O 4~6g / L, Na 2 SO 4 3~5g / L, (NH 4 ) 2 SO 4 4~6g / L,KH 2 PO 4 2~4g / L, KCl 1~2g / L, sodium glutamate 18~20g / L.

[0032] Insert the bacteria into the seed medium with 1% inoculum, cultivate for 24-48 hours, and the rotation speed is 170 rpm to complete the first-generation activation, and use the same method to activate the second-generation seeds to activate For three generations, the seeds were obtained.

[0033] (2) Fermentation culture

[0034] The components of the fermentation medium are: glucose 80-100g / L, yeast powder 4-6g / L, MgSO 4 ·7H 2 O 3~5g / L, Na 2 SO 4 6~9g / L, (NH 4 ) 2 SO 4 2~4g / L,KH 2 PO 4 2~4g / L, KCl 0.1~0.5g / L, NaCl 5~8g / L, sodium glutamate 18~20g / L.

[0035] The seed solution i...

Embodiment 3

[0037] This embodiment specifically illustrates the comparison of the shake flask fermentation performance of domesticated bacterial strains and original bacterial strains

[0038]Select the strains with excellent growth at 35°C, adopt the fermentation method of Example 2, and ferment at 28°C, 31°C and 35°C respectively, to compare the fermentation performance of different domesticated strains and original strains at normal temperatures ( DHA production capacity). The fermentation time was recorded, and the dry cell weight, fatty acid production and DHA content were measured after the fermentation was completed. The comparison of fermentation parameters between different domesticated strains and original strains is shown in Table 1.

[0039] Table 1 Comparison of fermentation parameters between the original strain and the domesticated strain at different temperatures

[0040]

[0041] The data in the table shows that under certain initial sugar conditions, the fermentatio...

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Abstract

The invention discloses a bacterial strain capable of producing DHA by high-temperature fermentation and its application. The bacterial strain can produce high-yield docosahexaenoic acid oil under the condition of 35°C. Strategies to obtain strains that can grow rapidly and accumulate DHA rapidly at high temperatures, and the growth rate of the strains of Schizochytrium after continuous high-temperature domestication has been greatly improved. When cultured at normal fermentation temperatures, the cell growth rate has increased by 75.43%. The fermentation period is shortened by 25h under high-temperature fermentation conditions, and the cell growth rate is 2.10 times that of the original strain. In addition, the yield of β-carotene of the strain increased by 57.75%. The bacterial strain of the present invention can also realize high-efficiency production of DHA at a high temperature of 35° C., reduces energy consumption in production in high-temperature areas, reduces fermentation costs, and is suitable for industrial production.

Description

technical field [0001] The invention relates to a bacterial strain capable of producing DHA by high-temperature fermentation and application thereof, belonging to the field of biotechnology. Background technique [0002] Docosahexaenoic Acid (DHA), an important omega-3 long-chain polyunsaturated fatty acid, commonly known as "brain gold", is one of the highly regarded functional oils. DHA mainly exists in the surface layer of neurons in the human brain, and plays an extremely important role in the regeneration of cell protrusions. Studies have shown that DHA can promote the development of the nervous system, effectively inhibit the incidence of senile dementia, reduce the probability of arteriosclerosis and thrombosis, and have physiological functions such as suppressing cancer, anti-inflammation, and improving the survival rate of aquatic products. [0003] The main sources of DHA are deep-sea fish oil and oleaginous microorganisms. Due to the advantages of microbial ferme...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P7/64C12R1/645
CPCC12N1/14C12P7/6427C12N1/145C12R2001/645
Inventor 任路静唐修阳毕志倩黄和胡学超
Owner NANJING TECH UNIV
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