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Method of extracting DNA from hair

A hair and debris technology, applied in the direction of DNA preparation, recombinant DNA technology, biochemical equipment and methods, etc., can solve the problems of long time consumption, low efficiency, damage to operators and the environment, and achieve high extraction efficiency, simple operation, Environmentally friendly effect

Inactive Publication Date: 2020-02-28
深圳市卫生健康发展研究中心
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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for extracting DNA from hair, which aims to solve the problem that the existing method for extracting DNA from hair needs to use harmful chemicals such as guanidine salts, which will cause harm to operators and the environment, and takes a long time. Low efficiency and other technical problems

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  • Method of extracting DNA from hair
  • Method of extracting DNA from hair
  • Method of extracting DNA from hair

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Embodiment 1

[0051] A method for extracting DNA from hair, comprising the steps of:

[0052] ①Take 3 hairs from the same person, name them A, B, and C respectively, cut off the hair roots within 1cm of the hair follicle end, and cut them into debris with a length of about 2-4mm, and collect each 0.2mL Then centrifuge at 2000×g for 15s to concentrate the debris at the bottom of the tube;

[0053] ② Add 20 μL of lysate to the PCR tube, then centrifuge at 2000×g for 15 s, then place it on a PCR instrument and set the temperature to 56°C for 20 min;

[0054] ③After the lysis is completed, concentrate the unlysed suspension at the bottom of the PCR tube by centrifugation at 2000×g for 15s, and then use a pipette with a volume range of 1-10 μL to transfer as much supernatant as possible to another new PCR tube , and finally add 36 μL of DNA purification magnetic beads to the PCR tube, centrifuge at 1000×g for 5 s to concentrate the components at the bottom of the tube, vortex and oscillate for ...

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Abstract

The invention belongs to the technical field of biology, and particularly relates to a method of extracting DNA from hair. The method includes steps: obtaining hair, and pretreating the hair to obtainhair fragments; obtaining lysate, and dispersing the hair fragments into the lysate for pyrolysis treatment to obtain a pyrolysis product; obtaining a magnetic bead suspension, adding the same the pyrolysis product, mixing, and separating to obtain a DNA solution in the hair, wherein the lysate is composed of Tris-HCl, NaCl, CaCl2, dithiothreitol, sodium dodecyl sulfate, protease and water. The method is quick and efficient in pyrolysis process, simple in operation, needless of toxic substances like phenol, chloroform and guanidine salt, friendly to operation personnel and environment, high in efficiency of extracting DNA in the hair, low in hair sample consumption, convenient in establishing automatic extraction methods afterwards and high in applicability.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for extracting DNA from hair. Background technique [0002] Since Bradfield and others successfully extracted nucleic acid from hair, hair has become one of the commonly used detection materials in forensic medicine and genetics. Compared with peripheral blood, it has the advantages of easy extraction, convenient transportation and storage, and high reproducibility. The DNA content is very small, and mainly exists around the hair root and sheath cells. According to reports, the hair root of freshly collected hair contains about 0.5 μg of total DNA, and the content in the hair shaft is even less. In addition, melanin in hair is a strong inhibitor in the PCR amplification process, which has a great impact on further downstream research and analysis. Therefore, it is particularly important to find a method that can effectively extract nucleic acids from hair. [0003] At prese...

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Application Information

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IPC IPC(8): C12N15/10
CPCC12N15/1013C12Q2523/308C12Q2527/125C12Q2523/32
Inventor 林圣郑开封陈仕国苏进娣高建段山
Owner 深圳市卫生健康发展研究中心
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