Personal care compositions comprising zinc:usnic acid complexes and methods of use
A personal care and composition technology, applied in the direction of washing composition containing organic compounds, drug combination, detergent composition, etc., can solve the problems that cannot fully solve the problems of water solubility and efficacy attributes
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example 4
[0089] Example 4 - Solubility Determination
[0090] Solubility determination of the Zn: lichenate complex was performed using standard techniques. The solubility of lichenic acid in deionized water at 35°C was reported to be 6.3 ppm (see Jin et al., Solubility of(+)-Usnic Acid in Water, Ethanol, Acetone, Ethyl Acetate and n-Hexane, J Solution Chem., 2013, 42:1018-1027). The Zn: lichenate complex showed a solubility of 13.5 ppm in deionized water at room temperature.
example 5
[0091] Example 5 - In Vitro Cup Scrub AB Test of Soap Bars
[0092] This assay measures residual antimicrobial efficacy. 1% of Zn: lichenate complex was added to alkaline soap flakes and processed into soap bars. Wet the strip sample and rub it against the pigskin. The pigskin is then rinsed, slightly dried and inoculated with Staphylococcus aureus. After incubation, recovered bacteria were collected using the cup scrubbing technique and counted using standard plate counting procedures. Average log counts of colony forming units (CFU) were calculated for replicate samples. As a placebo, alkaline soap flakes were processed into thin bars. As a positive control, will also have sodium palmitate, sodium oleate, sodium laureth (sodium soap), water, glycerin, talc, PEG-12, fragrance, sodium chloride, triclocarban, citric acid, A soap formulation of titanium dioxide, pentasodium pentetate, etidronate, yellow 10, orange 4 and also containing 0.4% triclocarban (TCC) was processed in...
example 6
[0095] Example 6-Minimum Inhibitory Concentration Determination
[0096] The minimum inhibitory concentration (MIC) is the lowest concentration of antimicrobial that inhibits the visible growth of microorganisms after overnight incubation. Determination of MIC values of zinc: lichenate complex and lichenic acid against Staphylococcus aureus and Escherichia coli by broth microdilution method. Briefly, 0.1% stock solutions were prepared in dimethylsulfoxide (DMSO). Using a 96-well microtiter plate, the corresponding test concentrations were 500, 250, 125, 62, 31, 16, 8, 4, 2, 1, 0.5 and 0.2 ppm. The level of bacterial growth was assessed using the optical density reading at 610 nm for each well. MIC results are listed in Table 2.
[0097] Table 2: MIC determination results
[0098]
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