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A synthetic method of tellurium nanorods based on genetically engineered polypeptides

A technology of tellurium nanorods and synthesis methods, applied in the field of synthesis of tellurium nanorods based on genetically engineered polypeptides, can solve the problems of difficult removal of chemical molecules, uneven shape, high toxicity, etc., and achieve low cost, low toxicity, and mild reaction Effect

Active Publication Date: 2021-09-21
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The general hydrothermal synthesis of tellurium nanoparticles requires the addition of some chemical reagents, such as PVP, SDS, etc., to maintain or improve the stability in aqueous solution, generally toxic and these chemical molecules are often difficult to remove during the application process; on the other hand , Only with an appropriate size can it be better applied in nanobiomedicine. Although there are many hydrothermal synthesis methods at present, they are relatively cumbersome, or the size is too large, or the shape is not uniform when the size is small.

Method used

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  • A synthetic method of tellurium nanorods based on genetically engineered polypeptides
  • A synthetic method of tellurium nanorods based on genetically engineered polypeptides
  • A synthetic method of tellurium nanorods based on genetically engineered polypeptides

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Experimental program
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Effect test

Embodiment 1

[0026] Tellurium nanorods polypeptide synthesis methods based on genetic engineering, comprising the steps of: 8ml of sodium tellurite solution (5 mM) and 1ml PC in 10ml round-bottomed flask 10 A protein solution (1mg / ml, with NaOH solution adjusted to pH 8), after vigorous stirring thoroughly mixed, then added rapidly a solution of sodium borohydride new configuration (10OmM) at 1ml, 30 ℃, rapid reaction was stirred 2h, dialysis (dialysis bag molecular weight 3500 Da) obtained after 6h tellurium nanorods.

Embodiment 2

[0028] Add 1ml of sodium tellurite in 10ml round bottom flask (2mM) and 1mg PC 10 Protein A (with NaOH solution adjusted to pH 8), mixed with vigorous stirring, then added rapidly to the new configuration sodium borohydride solution (10OmM) at 200μl, 30 ℃, rapid reaction was stirred 4h, dialysis (dialysis bag molecular weight after 3500Da) 6h tellurium nanorods obtained.

Embodiment 3

[0030] Add 1ml of sodium tellurite in 10ml round bottom flask (2mM) and 1mg PC 10 Protein A (with NaOH solution adjusted to pH 8), mixed with vigorous stirring, then added rapidly to the new configuration sodium borohydride solution (10OmM) at 200μl, 30 ℃, rapid reaction was stirred 4h, dialysis (dialysis bag molecular weight after 3500Da) 6h tellurium nanorods obtained.

[0031] Take 20μl present application prepared tellurium nanorods dropwise copper mesh obtained using transmission electron microscopy tellurium nanorods were characterized by transmission electron microscopy spectrum (TEM) see figure 1 , It found: tellurium nanorods prepared diameter of 4 ~ 6nm, a length of 20 ~ 30nm, good dispersibility. Take 200μl detection UV-vis spectroscopy, figure 2 Showed consistent absorption auxiliary agent and conventional chemical synthesis tellurium rod. image 3 X-ray diffraction pattern, the purified solution was lyophilized to a powder by 09empyrean x- ray diffractometer analysis, ...

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Abstract

The invention belongs to the field of nanomaterial synthesis, and in particular relates to a method for synthesizing tellurium nanorods based on genetically engineered polypeptides. The synthesis method includes: dissolving sodium tellurite powder in water to form solution A; dissolving the genetically engineered polypeptide in water, adjusting the pH to be alkaline, and dissolving it completely to form solution B; fully dissolving solution A and solution B After mixing, add newly prepared sodium borohydride solution to obtain solution C, and after a period of reaction, dialyze solution C to obtain tellurium nanorods. The present invention uses genetically engineered polypeptides instead of chemical molecular stabilizers, and the tellurium nanorod nanoparticles synthesized at room temperature have better biocompatibility and lower toxicity; the genetically engineered polypeptide PC of the present invention 10 A on the one hand provides guidance for the growth of tellurium nanorods, on the other hand PC 10 A nanogel structure limits the excessive growth of tellurium nanorods, and the synthesized tellurium nanorods are small in size and good in uniformity.

Description

Technical field [0001] The present invention belongs to the field of nano-synthetic material, particularly relates to a synthetic method based on tellurium nanorods genetically engineered polypeptide. Background technique [0002] Tellurium as the closest non-metallic material having a metallic nature, which itself has an excellent photoelectric and thermoelectric properties, as compared to the low toxicity of heavy metals, is easy to form functionalized metal compound such as cadmium telluride, silver telluride quantum point, so that in the field of electrochemistry, optoelectronics, nanotechnology and medicine has broad prospects. Hydrothermal synthesis generally tellurium nanoparticles need to add some chemical agent, such as PVP, SDS or the like to maintain or improve the stability in an aqueous solution, and general toxicity of these chemicals in the application process is often difficult to clean and remove molecules; on the other hand only appropriate size in order to bett...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C01B19/02B82Y40/00
CPCB82Y40/00C01B19/02
Inventor 刘波李朝卿赵元弟
Owner HUAZHONG UNIV OF SCI & TECH
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