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rice osrr22 -7 Mutant gene and identification method thereof, kasp typing primer for identification and application

A technology for typing primers and mutants, applied in applications, genetic engineering, plant genetic improvement, etc., can solve the problem of low mutation efficiency of target genes, improve accuracy and efficiency, and save time and cost.

Active Publication Date: 2021-08-17
HUNAN HYBRID RICE RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Based on the irreplaceable advantages of mutagenesis breeding, we hope to solve the problems of selection of beneficial mutations and low efficiency of target gene mutations

Method used

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  • rice osrr22 <sup>-7</sup> Mutant gene and identification method thereof, kasp typing primer for identification and application
  • rice osrr22 <sup>-7</sup> Mutant gene and identification method thereof, kasp typing primer for identification and application
  • rice osrr22 <sup>-7</sup> Mutant gene and identification method thereof, kasp typing primer for identification and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] A rice OsRR22 -7 The mutant gene has the nucleotide sequence shown in SEQ ID NO.1. According to the test results of Beijing Qingke Xinye Biotechnology Co., Ltd., the rice OsRR22 -7 The mutant gene is the deletion genotype of the 5th exon [CGGCTTT / -] of the rice OsRR22 gene [CGGCTTT / -] 7 bp (position 4140861-4140867).

[0073] a kind of like figure 1 As shown, identify whether the above rice OsRR22 is contained in the physical and chemical mutagenesis samples -7 A method for a mutant gene, comprising the steps of:

[0074] 1. Use 80MeV / u carbon ion ( 12 C 6+ ) with a dose of 180Gy to irradiate 20,000 seeds of rice variety 638S to obtain the M1 generation of rice variety 638S (hereinafter referred to as 638S).

[0075] 2. Plant 20,000 seedlings of the 638S M1 generation in individual plants. Take 96 plants as a group, each group has 8 rows, and each row has 12 plants, 100 groups are planted, a total of 9600 plants, and each group is numbered 1-100.

[0076] 3. Taki...

Embodiment 2

[0094] A rice OsRR22 -7 The mutant gene or the rice OsRR22 obtained in Example 1 -7 The application of the mutant of the mutant gene in breeding and preparing a salt-tolerant phenotype rice variety specifically includes the following steps:

[0095] 1) OsRR22 obtained with embodiment 1 -7 The mutant of the genetic phenotype of the mutant gene is used as the donor parent, and the rice Huazhan is used as the recipient parent to carry out cross breeding to obtain F1 seeds;

[0096] 2) After the F1 seed population is sown, a single plant is selected for hybridization with the recurrent parent rice Huazhan to obtain BC1F1 seeds;

[0097] 3) Sowing BC1F1 seeds, extracting BC1F1 population DNA from individual plants, and using the KASP typing primers designed in Example 1 to mark foreground selection, and to select rice containing OsRR22 -7 For the single plant of the mutant gene, background selection is performed, and the single plant with the highest background similarity to the...

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Abstract

The invention discloses a rice OsRR22 ‑7 Mutant gene and identification method thereof, the OsRR22 ‑7 Compared with the Nipponbare rice OsRR22 gene sequence, the mutant gene contains the following base deletion segment, namely: there is a [CGGCTTT / ‑]7 bp deletion at position 4140861‑4140867 (RAP_Locus). Simultaneously disclosed rice OsRR22 ‑7 The application of mutant genes in molecular assisted breeding of crops and in the selection or preparation of salt-tolerant phenotype rice varieties has achieved remarkable technical results. The invention also discloses a method for detecting, screening or obtaining rice OsRR22 ‑7 The KASP typing primers for mutants of mutant genes can accurately type the SNP / Indel found by high-depth targeted sequencing in different individuals, further improving the accuracy and efficiency of existing innovative germplasm screening and identification.

Description

technical field [0001] The invention belongs to the technical field of rice mutation breeding, and in particular relates to a rice mutant gene and an identification method, and also relates to identification KASP typing primers and related applications. Background technique [0002] Among the cereal crops that are propagated by seeds, wheat and rice are the main food crops in my country. With the rapid development of the national economy and society, the contradiction between population growth and arable land reduction has become increasingly prominent. my country has a large area of ​​soil with varying salinity, and cultivating cereal crops with improved salt tolerance can increase my country's arable land area and increase total grain output. [0003] Among cereal crops, rice has poor salt tolerance, and it is urgent to breed new varieties with improved salt tolerance through genetic improvement. Usually, mutagenesis is used to screen mutant materials with improved salt ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/11C12Q1/6858C12Q1/6895A01H1/02A01H1/04
CPCA01H1/02A01H1/04C07K14/415C12Q1/6858C12Q1/6895C12Q2600/156C12Q2535/122
Inventor 赵炳然余丽霞韶也李文建毛毕刚杜艳胡远艺唐丽彭彦李曜魁张丹袁隆平
Owner HUNAN HYBRID RICE RES CENT