A kind of etomidate nano preparation and preparation method thereof
A technology of etomidate and nanoliposomes, applied in nanotechnology, nanotechnology, nanomedicine, etc., can solve the problems of unusable and unfavorable preservation of nutrients, and achieve easy storage, non-irritating allergies, and biocompatibility good sex effect
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Embodiment 1
[0032] Embodiment 1 etomidate nano-preparation of the present invention
[0033] (1) Weigh 100mg of lecithin and 10mg of etomidate and put them in two 3ml test tubes, numbered a and b.
[0034] (2) Add 1ml of chloroform to the test tubes a and b respectively, shake well, and shake well until there are no solid particles left on the tube wall.
[0035] (3) Pour all the solutions in 2 test tubes a and b into a rotary evaporator at 25° C., keep a constant speed of 60 r / min, remove volatile solvents, and make the liposomes form a lipid dry film on the wall of the spherical device.
[0036] (4) After the thin film is formed, continue to flow nitrogen gas for 10 minutes to remove the residual volatile organic solvent.
[0037] (5) Add 5ml of phosphate buffer solution (PBS) with a pH value of 7.35, shake and shake well until there are no residual solid particles on the tube wall. After being completely dissolved, a phosphate buffer solution with a concentration of 2 mg etomidate pe...
Embodiment 2
[0039] Embodiment 2 etomidate nano-preparation of the present invention
[0040] (1) Weigh 90mg of lecithin, 10mg of pluronic P123, and 10mg of etomidate, and place them in three 3ml test tubes, numbered a, b, and c.
[0041] (2) Add 1ml of chloroform to the test tubes a, b, and c respectively, shake well, and shake well until there are no solid particles left on the tube wall.
[0042] (3) Pour all the solutions in the 3 test tubes a, b, and c into a rotary evaporator, keep a constant speed of 60r / min at 25°C, remove the volatile solvent, and make the liposomes form a lipid dry film on the wall of the spherical device.
[0043] (4) After the thin film is formed, continue to flow nitrogen gas for 10 minutes to remove the residual volatile organic solvent.
[0044] (5) Add 5ml of phosphate buffer solution (PBS) with a pH value of 7.35, shake and shake well until there are no solid particles remaining on the tube wall. After it is completely dissolved, a phosphate buffer solut...
Embodiment 3
[0046] Embodiment 3 etomidate nano-preparation of the present invention
[0047] (1) Weigh 80mg of lecithin, 20mg of Pluronic P123, and 10mg of etomidate in three 3ml test tubes, numbered a, b, c
[0048] (2) Add 1ml of chloroform to the test tubes a, b, and c respectively, shake well, and shake well until there are no solid particles left on the tube wall.
[0049] (3) Pour all the solutions in the 3 test tubes a, b, and c into a rotary evaporator, keep a constant speed of 60r / min at 25°C, remove the volatile solvent, and make the liposomes form a lipid dry film on the wall of the spherical device.
[0050] (4) After the thin film is formed, continue to flow nitrogen gas for 10 minutes to remove the residual volatile organic solvent.
[0051] (5) Add 5ml of phosphate buffer solution (PBS) with a pH value of 7.35, shake and shake well until there are no residual solid particles on the tube wall. After it is completely dissolved, a phosphate buffer solution with a concentrati...
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