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Preparation method and application of γ-polyglutamic acid gel nanoparticles

A technology of polyglutamic acid gel and polyglutamic acid, which is applied in skin care preparations, medical preparations without active ingredients, and medical preparations containing active ingredients, etc., can solve the problems of poor mechanical properties and liquid absorption capacity problems such as decline, to achieve the effect of good film formation, improved solubility and good stability

Active Publication Date: 2022-04-15
浙江坤澎科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, it is generally found in use that γ-polyglutamic acid hydrogel becomes weak after absorbing water and has poor mechanical properties, which will have great limitations in application.
In addition, γ-polyglutamic acid hydrogel can absorb water thousands of times for pure water, but in an ionic solution environment such as saline, the hydrogel’s liquid absorption capacity will be greatly reduced

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A gamma-polyglutamic acid gel nanoparticle prepared by the following method:

[0026] The first step, preparation of sodium tripolyphosphate solution: get sodium tripolyphosphate and drop into deionized water to prepare a 1.5 mg / ml sodium tripolyphosphate solution and adjust the pH to 6.0 with 0.3% (v / v) organic acid solution, slightly Pore ​​membrane filtration for standby;

[0027] The second step, preparation of chitosan solution: choose chitosan with molecular weight of 50kDa and drop into 0.3% (v / v) organic acid solution to be prepared into 2mg / ml chitosan solution, stir and swell for 16h, take the chitosan after swelling Adjust the pH of the sugar solution to 6.0 with a 0.08mol / L alkali solution, and filter through a microporous membrane for subsequent use;

[0028] The third step, preparation of γ-polyglutamic acid-polyvinylpyrrolidone solution: Weigh 300g γ-polyglutamic acid and 15g polyvinylpyrrolidone into 1L deionized water to prepare γ-polyglutamic acid-pol...

Embodiment 2

[0034] A gamma-polyglutamic acid gel nanoparticle prepared by the following method:

[0035] The first step, sodium tripolyphosphate solution preparation: get sodium tripolyphosphate and drop into deionized water and be mixed with the sodium tripolyphosphate solution of 2.5mg / ml and adjust pH with 0.5% (v / v) organic acid solution to be 6.5, slightly Pore ​​membrane filtration for standby;

[0036] The second step, preparation of chitosan solution: choose chitosan with molecular weight of 90kDa and put it into 0.5% (v / v) organic acid solution to be mixed with chitosan solution of 3mg / ml, stir and swell for 20h, take chitosan after swelling Adjust the pH of the sugar solution to 6.5 with a 0.1mol / L alkaline solution, and filter it through a microporous membrane for subsequent use;

[0037] The third step, preparation of γ-polyglutamic acid-polyvinylpyrrolidone solution: Weigh 400g γ-polyglutamic acid and 25g polyvinylpyrrolidone into 1L deionized water to prepare γ-polyglutamic...

Embodiment 3

[0043] A gamma-polyglutamic acid gel nanoparticle prepared by the following method:

[0044] The first step, preparation of sodium tripolyphosphate solution: get sodium tripolyphosphate and drop into deionized water to be prepared with 2.0mg / ml sodium tripolyphosphate solution and adjust the pH to 6.2 with 0.4% (v / v) organic acid solution, slightly Pore ​​membrane filtration for standby;

[0045]The second step, preparation of chitosan solution: choose chitosan with molecular weight of 60kDa and put it into 0.4% (v / v) organic acid solution to be mixed with chitosan solution of 2.5mg / ml, stir and swell for 18h, take the shell after swelling Adjust the pH of the polysaccharide solution to 6.2 with 0.09mol / L alkaline solution, and filter it through a microporous membrane for later use;

[0046] The third step, preparation of γ-polyglutamic acid-polyvinylpyrrolidone solution: Weigh 350g γ-polyglutamic acid and 20g polyvinylpyrrolidone into 1L deionized water to prepare γ-polyglut...

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Abstract

The present invention relates to a preparation method of γ-polyglutamic acid gel nanoparticles: in the first step, take sodium tripolyphosphate and put it into deionized water to prepare sodium tripolyphosphate solution, adjust the pH to 6.0-6.5 with organic acid solution, and filter for later use The second step, preparation of chitosan solution: select chitosan and drop into organic acid solution to prepare chitosan solution, stir and swell for 16-20h, get the swollen chitosan solution to adjust pH to 6.0-6.5, filter for subsequent use ; The third step, gamma-polyglutamic acid-polyvinylpyrrolidone solution preparation: claim that gamma-polyglutamic acid and polyvinylpyrrolidone are dropped into deionized water and be mixed with gamma-polyglutamic acid-polyvinylpyrrolidone solution and adjust the pH to 5.8-6.2, filter for later use; the fourth step, preparation of γ-polyglutamic acid nanoparticles: mix γ-polyglutamic acid-polyvinylpyrrolidone solution with sodium tripolyphosphate solution and add dropwise to the chitosan solution until Visible blue opalescence appears. In the fifth step, the γ-polyglutamic acid nanoparticles prepared in the fourth step are freeze-dried into nanoparticle dry powder.

Description

technical field [0001] The invention relates to a preparation method and application of gamma-polyglutamic acid gel nanoparticles, belonging to the technical field of gel processing. Background technique [0002] γ-polyglutamic acid (γ-PGA) is a polymer compound prepared by Bacillus subtilis through fermentation technology. a polypeptide molecule. It has a highly active -COOH group in its molecular chain, which has been found to have many usable functions, such as water absorption, moisture retention and adsorption of heavy metals. Pollution, is an environmentally friendly material. Because γ-polyglutamic acid contains more carboxyl groups and active groups, it has strong water retention and slow release properties. These two properties make it widely used in health care products, pharmaceuticals and cosmetics. [0003] However, it is generally found in use that γ-polyglutamic acid hydrogel becomes weak after absorbing water and has poor mechanical properties, which will ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/12C08L77/04C08L5/08C08L39/06C08K3/32A61K8/02A61K8/88A61K8/9789A61K9/19A61K36/185A61K47/34A61P17/16A61Q19/00A61Q19/02
CPCC08J3/12A61K9/19A61K47/34A61K36/185A61P17/16A61K8/0283A61K8/88A61K8/9789A61Q19/00A61Q19/02C08J2377/04C08J2405/08C08J2439/06
Inventor 余作龙陶杨匀徐平
Owner 浙江坤澎科技有限公司