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Method for determining concentration of glimepiride in blood plasma by liquid chromatography-mass spectrometry

A technology of liquid chromatography-mass spectrometry and glimepiride, which is applied in the field of medicine, can solve the problems of no glimepiride determination method, literature reports, etc., and achieves the effects of simple pretreatment method, good peak shape and stable baseline.

Inactive Publication Date: 2020-03-27
徐州立兴佳正医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no literature report on the determination method of glimepiride

Method used

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  • Method for determining concentration of glimepiride in blood plasma by liquid chromatography-mass spectrometry
  • Method for determining concentration of glimepiride in blood plasma by liquid chromatography-mass spectrometry
  • Method for determining concentration of glimepiride in blood plasma by liquid chromatography-mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0031] Example: Human K 2 Determination of Glimepiride Concentration in EDTA Plasma

[0032] 1. Experimental materials and analytical equipment

[0033] Glimepiride (analyte): Toronto Research Chemicals or equivalent or higher grade standard

[0034] Glimepiride-d4 (internal standard): TLC Pharmaceutical Standards or equivalent or higher grade standard

[0035] The reagents used are shown in Table 1 below:

[0036] Table 1 Reagent Details

[0037] Reagent name level manufacturer Acetonitrile HPLC J. T. Baker Methanol HPLC J. T. Baker formic acid ACS Adamas

[0038] Note: Reagents of the same grade or higher can also be used

[0039] The analytical equipment used is shown in Table 2 below:

[0040] Table 2 Details of equipment used

[0041] components type manufacturer Binary pump (binary pump) AC Pump AB SCIEX Degasser Degasser AB SCIEX Column oven AC Column oven AB SCIEX Autosamp...

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Abstract

The invention discloses a method for determining the concentration of glimepiride in blood plasma by liquid chromatography-mass spectrometry, which adopts a liquid chromatography-mass spectrometry system for determination and comprises the following steps: taking a sample to be determined, adding a certain amount of mixed organic solvent for extraction and pretreatment, performing separating by achromatographic column, and performing detecting by using a mass spectrometry detector. The method disclosed by the invention is rapid, accurate, high in sensitivity and simple and convenient to operate, and provides a basis for measuring the blood concentration of glimepiride; the linear range of a plasma standard curve of the method is 0.5-500 ng / mL, the intra-batch precision RSD and the inter-batch precision RSD are both smaller than + / -15%, and the method is suitable for measuring the concentration of glimepiride in plasma.

Description

technical field [0001] The invention belongs to the technical field of medicines, in particular to a method for determining a drug, in particular to a method for determining the concentration of glimepiride in blood plasma by liquid mass spectrometry. Background technique [0002] Glimepiride is a sulfonyl hypoglycemic drug developed by the German company Hoechst Marion Roussel in the 1980s and first launched in Germany. Glimepiride is a third-generation sulfonylurea antidiabetic drug, and its mechanism of action is through binding to the sulfonylurea receptor (a protein with a relative molecular mass of 6.5×104) on the surface of pancreatic β-cells. The ATP-sensitive K+ (KATP) channel is coupled to promote the closure of the KATP channel, causing the depolarization of the cell membrane and the opening of the voltage-dependent calcium channel. 2+ Influx promotes the release of insulin and inhibits hepatic glucose synthesis. Recent studies have shown that different sulfonyl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06G01N30/34G01N30/72
CPCG01N30/06G01N30/34G01N30/72G01N30/88G01N2030/045G01N2030/062G01N2030/8822G01N2030/884
Inventor 林明弘
Owner 徐州立兴佳正医药科技有限公司
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