Centrifugal micro-fluidic chip and system for SAT and detection method
A microfluidic chip and centrifugal technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, specific-purpose bioreactor/fermenter, etc., can solve the needs of rapid and low-cost clinical diagnosis , cumbersome operation, time-consuming sample preparation, etc., to achieve the effect of rapid acquisition, improved detection efficiency, and simple structure
Pending Publication Date: 2020-03-31
深圳市华迈生物医疗科技有限公司
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Problems solved by technology
However, like conventional nucleic acid detection techniques, existing devices and methods for implementing SAT cannot get rid of the shortcomings of time-consuming sample preparation and cumbersome operations, and do not meet the needs of rapid and low-cost clinical diagnosis
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[0028] Embodiments of the present invention will be described in detail below. It should be emphasized that the following description is only exemplary and not intended to limit the scope of the invention and its application.
[0029] It should be noted that when an element is referred to as being “fixed” or “disposed on” another element, it may be directly on the other element or be indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or indirectly connected to the other element. In addition, connection may be for both a fixed function and a circuit / signal communication function.
[0030] It is to be understood that the terms "length", "width", "top", "bottom", "front", "rear", "left", "right", "vertical", "horizontal", "top" , "bottom", "inner", "outer" and other indicated orientations or positional relationships are based on the orientations or positional relationships sh...
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The invention discloses a centrifugal micro-fluidic chip and system for SAT and a detection method. The chip comprises a substrate layer and a cover plate layer which are stacked together. The substrate layer is provided with a lower sample injection inlet, a lower storage chamber, a lower air outlet, a clockwise flow channel, a constant-temperature amplification chamber lower part, a first counterclockwise flow channel and a liquid outlet. The cover plate layer is provided with an upper liquid inlet, an upper storage chamber, an upper air outlet, a second counterclockwise flow channel and a constant-temperature amplification chamber upper part; reaction liquid is stored in the lower storage chamber; the lower storage chamber is respectively connected with the lower sample injection inletand the lower gas outlet and is connected with the lower part of the constant-temperature amplification chamber through the clockwise flow channel; and the lower portion of the constant-temperature amplification chamber is connected with the liquid outlet through the first counterclockwise flow channel. The upper storage chamber stores cleaning liquid; the upper storage chamber is connected with the upper liquid inlet and the upper air outlet and is connected with the upper portion of the constant-temperature amplification chamber through the second counterclockwise flow channel; and the constant-temperature amplification chamber is provided with a transparent area. According to the invention, amplification and detection of nucleic acid can be completed rapidly, stably and reliably.
Description
technical field [0001] The invention relates to the technical field of molecular diagnosis, in particular to a centrifugal microfluidic chip and a detection method for SAT (real-time fluorescent nucleic acid constant temperature amplification detection). Background technique [0002] The concept of micro total analysis system was first proposed in the 1990s. Since then, on the basis of microelectronics, micromechanics, bioengineering and nanotechnology, microfluidic technology has developed rapidly and has become one of the most cutting-edge scientific and technological fields in the world. At present, its core technology is a microfluidic chip based on microfluidic technology, also known as a lab on chip (Lab on chip). Microfluidic chips are widely used in the biomedical field because of their advantages such as low consumption, low cost, high throughput, and automatic operation. One of the important applications is molecular diagnostic technology based on microfluidic chip...
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IPC IPC(8): C12M1/00C12M1/34C12Q1/6844
CPCC12Q1/6844C12Q2531/119C12Q2563/107C12Q2565/629C12Q2561/113
Inventor 弥胜利杨伟豪彭继炜黄嘉骏
Owner 深圳市华迈生物医疗科技有限公司