Method for efficiently and biologically synthesizing nano ferrous sulfide SM-FeS material, synthesized nano ferrous sulfide SM-FeS material and applications of the material
A technology of biosynthesis and nano-sulfur, which is applied in the field of environmental microbial materials, can solve the problems of increasing cost and unstable biosynthesis process, and achieve the effect of reducing the requirement of low tolerance, shortening the synthesis cycle, and improving the rate and stability
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Embodiment 1
[0054] A method for efficient biosynthesis of nano-iron sulfide SM-FeS material, comprising the following steps:
[0055] (1) 0.1g / L NaHCO 3 , 0.1g / L KH 2 PO 4, , 0.05g / L CaCl 2 ·H 2 O, 1g / L NH 4 Cl, 0.1g / LNaCl, 0.1g / L MgCl 2 ·6H 2 O, yeast extract 1 g / L, sodium lactate 5.4 g / L, 100 mM HEPES were dissolved in 950 ml of deionized water to obtain a modified M641 liquid medium, and the pH of the modified M641 liquid medium was adjusted to 7.0 with 1 M NaOH. Aliquot 50ml of modified M641 liquid medium into 100ml anaerobic bottles. Blow off with nitrogen for 30 minutes to remove dissolved oxygen in the liquid, then plug it with a butyl rubber stopper and cover with an aluminum cover, and sterilize at 121 °C for 30 minutes. After cooling to room temperature, take FeSO 4 ·7H 2 O solution and Na 2 S 2 O 3 The solution was poured into an anaerobic bottle through a 0.22 μm filter membrane, and in the mixture of the anaerobic bottle, FeSO 4 ·7H 2 The concentration of O was...
Embodiment 2
[0066] Set the mixed bacteria group SM and the single bacteria group DM to three parallel and one blank respectively, the medium used is the compound medium described in step (1) described in step (1), the inoculation conditions are exactly the same as in Example 1, and the blank has no Inoculate bacteria.
[0067] The mixed bacteria group SM is basically the same as that of Example 1, the only difference is that the incubation time of the constant temperature shaking shaker in step (4) is 0, 1, 2, 3, 5, and 7 days.
[0068]The single bacteria group DM is basically the same as the comparative example 1, the only difference is that the incubation time of the constant temperature shaking shaker in step (4) is 0, 1, 2, 3, 5, and 7 days.
[0069] Samples were taken on the 0th, 1st, 2nd, 3rd, 5th, and 7th days, and after passing through a 0.22 μm filter membrane, the samples were diluted 10 times, and the content of lactate was determined by high performance liquid phase.
[0070]...
Embodiment 3
[0072] (1) respectively prepare the solution that the hexavalent chromium concentration is 50ppm and 100ppm, pH=5.0; Wherein, carry out pH adjustment with the phthalic acid buffer solution of 50mM, adjust the pH value to be 5.0; The background ion concentration is 0.1M NaCl;
[0073] (2) get respectively the solution that 2 parts of hexavalent chromium concentrations are 50ppm and the solution that 2 parts of hexavalent chromium concentrations are 100ppm; 2 parts of hexavalent chromium concentrations are the solutions of 50ppm, one part will add the solution obtained in Example 1 The nano-iron sulfide SM-FeS material is used as the experimental group; the other part is not added, which is used as a blank control group; two parts of the solution with a hexavalent chromium concentration of 100ppm, one part will be added with the nano-iron sulfide prepared in Example 1. SM-FeS material was used as the experimental group, and the other was not added as the blank control group;
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