Check patentability & draft patents in minutes with Patsnap Eureka AI!

Mesenchymal stem cell compound active factor freeze-dried powder and its preparation method and application

A technology of active factors and mesenchymal stem cells, which is applied in the direction of cell culture active agents, biochemical equipment and methods, animal cells, etc., can solve the problems of component extraction and purification that cannot be completely removed, high risk, infection, etc., to promote division and Effects of proliferation, promotion of metabolism, and promotion of angiogenesis

Active Publication Date: 2020-12-08
广州赛加生物科技有限公司
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most media contain commercially available fetal bovine serum or chemical components that are not suitable for the human body. For example, fetal bovine serum may carry unknown pathogens or foreign proteins such as prion protein. Once it enters the human body, it may cause infection and allergy and other adverse events, the risk is high, and these components cannot be completely removed in the process of extraction, purification, and concentration, so it is very restrictive in clinical application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Mesenchymal stem cell compound active factor freeze-dried powder and its preparation method and application
  • Mesenchymal stem cell compound active factor freeze-dried powder and its preparation method and application
  • Mesenchymal stem cell compound active factor freeze-dried powder and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0052] In Example 2, the commercially available serum-free complete medium for adipose stem cells was purchased from Celera Stem Cell Technology Co., Ltd., and the product number is S-10-012;

[0053] MEM-α medium was purchased from Shanghai Yuanpei Biotechnology Co., Ltd., the product number is L562KJ.

Embodiment 1

[0054] Example 1: Extraction and phenotypic identification of adipose stem cells

[0055] Take excess fat from healthy volunteers after liposuction, add excess sterile double-antibody-containing PBS buffer, soak for 10-15 minutes, then centrifuge at 2000 rpm for 10 minutes, remove blood and PBS buffer, and repeat once. Transfer the obtained fresh adipose tissue to a 50ml centrifuge tube, add an equal volume of 0.075% collagenase and 0.25% trypsin (1:1), and digest in a water bath at 37°C for 40-50min. After the liquid was chylus, it was centrifuged at 1000rpm for 5min and filtered with a 200-mesh cell sieve. 2 , 95% air, incubate in a cell incubator at 37°C, observe the growth status of the cells the next day, and perform a half-change or full-change of the medium. When the cell confluence reaches more than 80%, use 0.25% trypsin~EDTA to digest the cells , to obtain primary adipose stem cells.

[0056] Among them, the preparation of the first medium is as follows: MEM-α medi...

Embodiment 3

[0063] Example 3: Effect of pre-coating treatment on the attachment performance of human-derived adipose-derived stem cells

[0064] Take 10 ml of pre-coating solution mixed with equal volumes of 10 μg / ml fibronectin and 0.001 g / ml polyvinyl alcohol (PVA), and gently add it to the 75cm 2 In the culture bottle, gently tap the culture bottle with your fingers to remove air bubbles to ensure that the pre-coating solution fully covers the bottom wall of the culture bottle, transfer it to a 4°C refrigerator, and place it for 30 minutes for later use.

[0065] Take 10 ml of 10 μg / ml fibronectin pre-coating solution, and gently add it along the wall of the culture flask to 75cm 2 In the culture bottle, gently tap the culture bottle with your fingers to remove air bubbles to ensure that the pre-coating solution fully covers the bottom wall of the culture bottle, transfer it to a 4°C refrigerator, and place it for 30 minutes for later use.

[0066] Take 10 ml of 0.001 g / ml polyvinyl alc...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to mesenchymal stem cell composite active factor freeze-dried powder as well as a preparation method and application thereof. In the preparation process of the composite active factor freeze-dried powder, a specific pre-coating solution is used for treating a cell culture container; and mesenchymal stem cells are cultured by adopting a first culture medium and a second culture medium under specific culture conditions, so the mesenchymal stem cells can be still kept at a normal state in the processes of hunger culture and induction and secrete more active factors. The composite active factor freeze-dried powder prepared by the invention can promote cell division and cell proliferation, repair damaged skin or skin with thin congenital cuticle, tighten the skin and reduce wrinkles; moreover, the immunoregulation function of the skin is activated, the metabolism of the skin is promoted, and an anti-aging effect is achieved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to freeze-dried powder of mesenchymal stem cell compound active factor and its preparation method and application. Background technique [0002] Mesenchymal stem cells (MSCs) are a type of mesenchymal stem cells (MSCs) derived from the mesoderm and ectoderm of the prenatal embryonic development stage, and exist in various tissues and organs, such as bone marrow, adipose tissue, muscle tissue, lung, liver, pancreas and synovium, etc. MSCs have multi-directional differentiation ability and paracrine function. Under the action of different inducing factors, they can differentiate into adipocytes, chondrocytes, and city cells; they can also secrete a variety of cytokines, including chemokines, adhesion molecules, etc. In recent years, studies have shown that various biologically active factors secreted by stem cells can be used as a new type of cell-free biological regulation mode to promo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/40A61K9/19A61P39/06A61K8/02A61K8/99A61K8/98A61K8/64A61K8/67A61Q19/08C12N5/0775A61K38/06A61K31/375A61K35/28
CPCA61K8/022A61K8/64A61K8/676A61K8/981A61K8/982A61K8/99A61K9/0014A61K9/19A61K31/375A61K35/28A61K38/06A61K38/40A61K2800/805A61Q19/08A61P39/06C12N5/0667C12N2500/24C12N2500/38C12N2501/105C12N2501/11C12N2501/115C12N2501/15C12N2501/91C12N2501/998A61K2300/00
Inventor 曾伙妹洪军辉马钦海陈永康谢文凤
Owner 广州赛加生物科技有限公司
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com