Glucose-responsive photonic crystal sensor, its preparation method and use method
A photonic crystal and responsive technology, applied in nanotechnology for sensing, color/spectral characteristic measurement, nanotechnology, etc., can solve the problems that response time hinders the development of glucose-responsive photonic crystal colorimetric sensors and limits applications, etc. Achieve the effects of easy operation, long response time and simple preparation method
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Embodiment 1
[0054] (1) Disperse superparamagnetic nanoparticles (consisting of ferric oxide nanocrystal cluster core and polyvinylpyrrolidone coated on its surface) into 3-acrylamidophenylboronic acid (AAPBA), N-(2- Hydroxyethyl) acrylamide (HEAA), photoinitiator 2-hydroxy-2-methyl-1-phenylacetone (HMPP), cross-linking agent methylenebisacrylamide (BIS), dimethyl sulfoxide and water Form the pre-poly solution in the solution; in the pre-poly solution: the concentration of AAPBA is 0.023mmol / mL, the concentration of HEAA is 0.20mmol / mL, the concentration of HMPP is 3% of the molar weight of AAPBA and HEAA monomer, and the concentration of BIS is The molar weight of AAPBA and HEAA monomers is 2%, the concentration of selected superparamagnetic nanoparticles is 1.0mg / mL, the particle diameter is 120nm, and the volume ratio of dimethyl sulfoxide to water is 100 / 900.
[0055] (2) Place the pre-polymerization solution under an external magnetic field of 250Gs, and after standing still for 2 min...
Embodiment 2
[0062] (1) Disperse superparamagnetic nanoparticles (consisting of iron ferric oxide nanocrystal cluster core and polyvinylpyrrolidone coated on its surface) into 3-acrylamidophenylboronic acid (AAPBA), acrylamide (AM) , photoinitiator 2-hydroxyl-2-methyl-1-phenylacetone (HMPP), crosslinking agent methylene bisacrylamide (BIS) and dimethyl sulfoxide and water to form a pre-poly solution; In the polymer solution: the concentration of AAPBA is 0.023mmol / mL, the concentration of AM is 0.20mmol / mL, the concentration of HMPP is 3% of the molar weight of AAPBA and AM monomer, and the concentration of BIS is 2% of the molar weight of AAPBA and AM monomer. %, the concentration of the selected superparamagnetic nanoparticles is 1.0 mg / mL, the particle diameter is 150 nm, and the volume ratio of dimethyl sulfoxide to water is 100 / 900.
[0063] (2) Place the pre-polymerization solution under an external magnetic field of 250Gs, and after standing still for 2 minutes, cure it with an ultr...
Embodiment 3
[0067] (1) Disperse the superparamagnetic nanoparticles (composed of ferric oxide nanocrystal cluster inner core and polyvinylpyrrolidone coated on its surface) into 3-acrylamidophenylboronic acid (AAPBA), photoinitiator 2- Hydroxy-2-methyl-1-phenylacetone (HMPP), cross-linking agent methylene bisacrylamide (BIS), dimethyl sulfoxide and water to form a prepolymer solution; in the prepolymer solution: AAPBA The concentration is 0.012mmol / mL, the concentration of HMPP is 5% of the molar weight of AAPBA monomer, the concentration of BIS is 2% of the molar weight of AAPBA monomer, and the concentration of selected superparamagnetic nanoparticles is 1.0 mg / mL. The diameter is 120nm, and the volume ratio of dimethyl sulfoxide to water is 400 / 600.
[0068] (2) Place the pre-polymerization solution under an external magnetic field of 250Gs, and after standing still for 2 minutes, cure it with an ultraviolet lamp for 5 minutes. After the reaction, wash the prepared photon nanochains wi...
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