Method for rapidly detecting growth process of cassava solid-state fermentation on line
A solid-state fermentation and growth process technology, which is applied in the direction of material excitation analysis, Raman scattering, etc., can solve the problems of one-sided detection technology, time-consuming and labor-intensive, etc.
Active Publication Date: 2020-04-10
JIANGSU UNIV
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[0005]
In view of the development of the prior art, the purpose of the present invention is to provide a rapid on-line method for detecting the growth process of cassava solid-state fermentation based on Raman spectroscopy combined with olfactory visualization technology, so as to solve the problems of time-consuming and labor-intensive conventional techniques and one-sided detection techniques.
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[0056] The present invention has versatility for the rapid detection of the fermentation process, but because there are many solid-state fermentation raw materials, this experiment only uses cassava solid-state fermentation as an implementation example, and other fermentation process detections can refer to the method of this implementation example, specifically for different raw materials The detection of the solid-state fermentation process can be realized by establishing a new discriminant model for the detection of the solid-state fermentation process.
[0057] The technical solutions implemented in the present invention will be described in detail below in conjunction with the accompanying drawings for implementing the present invention.
[0058] The experimental flow design of the solid-state fermentation of cassava is as follows: First, the cassava was washed and peeled, and then dried in an electric heating constant temperature blast drying oven at 70 °C to a constant w...
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Abstract
The invention discloses a method for rapidly detecting the growth process of cassava solid-state fermentation on line. The method comprises the following specific steps: taking a batch of cassava andcarrying out solid-state fermentation on the cassava; and detecting the content of glucose by using a DNS solution, detecting the content of ethanol by using a potassium dichromate solution, and measuring standard curves of glucose and ethanol; respectively carrying out Raman spectrum and smell visual detection on a cassava solid-state fermentation system in a sealed lightproof room, respectivelyextracting Raman spectrum characteristic variables and smell visual characteristic variables, carrying out characteristic-level fusion, establishing a cassava solid-state fermentation process detection qualitative model, and comparing the cassava solid-state fermentation process detection qualitative model with the previous two single technologies. The method for rapidly detecting the growth process of cassava solid-state fermentation on line based on the Raman spectrum combined with the olfaction visualization technology is established, the stage of solid-state fermentation can be comprehensively and accurately judged, a guiding effect is provided for industrial fermentation, the cost is saved, and the fermentation yield is increased.
Description
technical field [0001] The invention relates to a method for real-time detection of the growth process of cassava solid-state fermentation, in particular to a rapid detection method based on the combination of Raman spectrum and olfactory visualization analysis technology, which is used for online detection of cassava solid-state fermentation in different stages. Background technique [0002] With the rapid development of science and technology and economy in our country, the issue of sustainable energy development has attracted more and more attention. As a renewable clean energy, the development of fuel ethanol has always been the focus of attention of countries all over the world. At present, the main production methods of ethanol are divided into liquid fermentation and solid state fermentation. Among them, the research on liquid fermentation technology and process detection sensors has been relatively mature. Solid-state fermentation does not require a strict operatin...
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IPC IPC(8): G01N21/65
CPCG01N21/65Y02E50/10
Inventor 江辉许唯栋陈全胜
Owner JIANGSU UNIV