Preparation method of nattokinase microcapsules
A technology of nattokinase and plant polyphenols, applied in biochemical equipment and methods, microcapsules, medical preparations of non-active ingredients, etc., can solve the problems of unstable microcapsules and low encapsulation efficiency of nattokinase, Achieve the effect of improving bioavailability, improving stability and improving encapsulation efficiency
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Embodiment 1
[0025] Step 1) Take 2 g of burnet, 3.2 g of 3-methyl fatty acid chloride, 4.8 g of sodium hydroxide, and 20 mL of dioxane, and react at 100° C. for 3 h under nitrogen protection. The product is dissolved in petroleum ether and washed with saturated brine. The petroleum ether was distilled off under reduced pressure to obtain the burnet-(3)-methyl fatty acid chloride complex, which can be dried and stored.
[0026] Step 2), according to the mass ratio of burletin-(3)-methyl fatty acid chloride complex, isopropyl myristate, nattokinase freeze-dried powder and water is 1:20:2:77, take step 1 3 g of the obtained product, 60 g of isopropyl myristate, 6 g of lyophilized nattokinase powder and 231 g of water were mixed and homogenized by an ultrasonic cell disruptor for 1 min to obtain a W / O emulsion embedding nattokinase.
[0027] Step 3), take 100mL of the emulsion and add it to the feeder of the molecular distillation equipment. In the high vacuum distillation method, the distilla...
Embodiment 2
[0029] Step 1), take 2g of cinnamon polyphenols, 4g of hexanoyl chloride, 4g of sodium hydroxide, and 20mL of dioxane, and react at 100°C for 4h under nitrogen protection. The product is dissolved in petroleum ether and washed with saturated brine, and the petroleum ether is removed by distillation under reduced pressure. The obtained cinnamon polyphenol-caproyl chloride complex can be dried and stored.
[0030] Step 2), according to the mass ratio of cinnamon polyphenol-caproyl chloride complex, triglyceride, nattokinase freeze-dried powder and water is 2:25:3:70, take the product obtained in step 1 6g, triglyceride 60 g, 9 g of nattokinase freeze-dried powder and 210 g of water were mixed and then homogenized by an ultrasonic cell disruptor for 1 min to obtain a W / O emulsion embedding nattokinase.
[0031] Step 3), take 100mL of the emulsion and add it to the feeder of the molecular distillation equipment. In the high vacuum distillation method, the distillation temperature ...
Embodiment 3
[0033] Step 1), take 1g of aloe-emodin, 3g of octanoyl chloride, 3.5g of sodium hydroxide, and 20mL of dioxane, react for 5h at 100°C under nitrogen protection, dissolve the product in petroleum ether and wash with saturated brine, and distill off the petroleum under reduced pressure Ether to obtain the emodin-octanoyl chloride complex, which can be dried and stored.
[0034] Step 2), according to the mass ratio of emodin-octanoyl chloride complex, corn oil, nattokinase lyophilized powder and water is 3:30:2:67, take 9g of the product obtained in step 1, 90g of triglyceride, sodium After mixing 6 g of soybean kinase freeze-dried powder and 201 g of water, it was homogenized by an ultrasonic cell disruptor for 1 min to obtain a W / O emulsion embedding nattokinase.
[0035] Step 3), take 100mL of the emulsion and add it to the feeder of the molecular distillation equipment. In the high vacuum distillation method, the distillation temperature is 80°C, the condensation surface temp...
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