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Anthocyanin composition with good stability

An anthocyanin and stability technology, which is applied in the directions of non-active ingredients medical preparations, medical preparations containing active ingredients, plant raw materials, etc., can solve problems such as instability, and achieve good stability, long half-life, and half-life prolonged effect

Pending Publication Date: 2020-04-21
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, anthocyanins belong to flavonoid polyphenols, and their special structure determines their strong instability

Method used

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  • Anthocyanin composition with good stability
  • Anthocyanin composition with good stability
  • Anthocyanin composition with good stability

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Extraction and content determination of anthocyanins from black fruit Lycium barbarum

[0027] Accurately weigh 5.0 g of black wolfberry into a brown reagent bottle, add 75 mL of 50% methanol, and perform ultrasonic extraction at 150 W, frequency of 20 kHz, and 70°C for 90 minutes to obtain the product.

[0028] The content of the anthocyanins obtained by the above extraction was measured by the pH differential method to be 16.24±0.16 mg / mL. The content of anthocyanin is measured by pH differential method: use distilled water as a blank control, and measure at 520nm and 700nm respectively. According to the content of anthocyanin in different samples, potassium chloride buffer solution (0.025mol / L, pH 1.0) is diluted into 10-100 times the sample solution. Similarly, at 520nm and 700nm, use sodium acetate buffer solution (0.4mol / L, pH 4.5) to dilute the sample by 10-100 times according to the content of anthocyanin in different samples. Note that it should be w...

Embodiment 2

[0037] Example 2 Extraction and content determination of berry polyphenolic compounds

[0038] Weigh 300g of fresh berries, use a cooking machine to crush and beat them, 60% ethanol according to the ratio of solid to liquid of 1:6.7, 45°C, 100W ultrasonic wave for 30min, centrifuge at 2500r / min for 10min, and collect the supernatant , the residue was repeatedly extracted 3 times in the same way, and the 3 extractions were combined and filtered to remove the excess residue, and concentrated in vacuo at 40°C using a rotary evaporator, and concentrated to 100 mL respectively to obtain the final product.

[0039] The above-mentioned berries are any one of indigo fruit, raspberry and black currant.

[0040]Determination of polyphenolic compounds extracted from berries: Soak AB-8 macroporous resin with 95% ethanol for 24 hours, then wash the resin with absolute ethanol until no white turbidity appears after adding water, and then rinse with distilled water until there is no alcohol ...

Embodiment 3

[0048] Preparation and treatment of embodiment 3 anthocyanin and polyphenol mixed solution

[0049] The polyphenolic compounds extracted in Example 2 were mixed with the anthocyanins extracted in Example 1 according to 1:0, 1:1, 1:5, 1:10, and 0:1, totaling 5 different concentration ratios, and the After the mixture was treated in 85°C water bath for 0h, 5h, 10h, 15h, 20h, 25h, 30h, 35h, the changes of total anthocyanin and anthocyanin monomer content in the mixture were detected by pH differential method and HPLC respectively. Wherein, the calculation formula is formula 1, and the determination of the content of anthocyanins refers to the determination method of AOAC anthocyanins, and the measurement wavelengths are 520 and 700 nm respectively.

[0050] The parameters of the HPLC detection method are:

[0051] Mobile phase: A-water solution containing 0.1% formic acid, B-acetonitrile solution containing 0.1% formic acid; flow rate: 1mL / min; detection wavelength: 520nm; chrom...

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Abstract

The invention discloses an anthocyanin composition with good stability, and relates to the technical field of plant extraction. The anthocyanin composition can prolong the half-life period of anthocyanin, so that the stability and the storage life of anthocyanin are improved. The anthocyanin composition with good stability is prepared from components including a polyphenol compound and lycium ruthenicum anthocyanin, wherein the polyphenol compound contains no sea-buckthorn polyphenol compound.

Description

technical field [0001] The invention relates to the technical field of plant extraction, in particular to an anthocyanin composition with good stability. Background technique [0002] Anthocyanins, also known as anthocyanins or anthocyanins, are a class of water-soluble natural pigments widely present in plants in nature. Most of the main coloring substances in fruits, vegetables, cereals, and dark flowering plants are related to it. Under the different pH conditions of plant cell vacuoles, anthocyanins make petals show colorful colors. Anthocyanins are mainly used in food coloring, dyes, medicine, cosmetics and so on. Anthocyanin is a strong antioxidant, which can prevent and alleviate the aging of the human body; anthocyanin can promote the regeneration of rhodopsin in retinal cells, prevent myopia, relieve eye fatigue, and improve vision; anthocyanin through Inhibition of elastase and collagenase makes the skin smooth and has anti-radiation effects; anthocyanins have t...

Claims

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Application Information

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IPC IPC(8): A61K36/815A61K47/46
CPCA61K36/815A61K47/46A61K2236/333A61K2236/39
Inventor 胡娜栾广祥王洪伦董琦周武
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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