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Drug-loaded synovial fluid additive and preparation method and application thereof

A technology for joint synovial fluid and additives, which is applied in the directions of drug combination, pharmaceutical formulation, antipyretics, etc., can solve the problem that the synthesis and preparation technology has not been established, achieve non-destructive loading and controllable release, solve the difficulty of surface modification, prolong the Effect of Lubrication Time

Active Publication Date: 2020-04-24
QUFU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no public report that fluorinated graphene is used to enhance hyaluronic acid, improve its mechanical properties, and use it to load anti-inflammatory drugs and treat joint inflammation, and the relevant synthesis preparation technology has not yet been established.

Method used

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  • Drug-loaded synovial fluid additive and preparation method and application thereof
  • Drug-loaded synovial fluid additive and preparation method and application thereof
  • Drug-loaded synovial fluid additive and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] (1) Prepare Tris-HCl buffered saline solution (TBS solution) with pH=8.5: Accurately weigh 0.12114 g of Tris-HCl solid, add 20 mL of ultrapure water to dissolve, and adjust the pH to 8.5 with concentrated HCl.

[0040] (2) Accurately weigh 1 g HA, dissolve 50 mg FGO in 100 mL TBS solution with pH=8.5, and sonicate for 40 min; add 500 mg DA-HCl, and adjust the pH value to 8.5 with HCl; the oxygen content of FGO is 45%, the fluorine content is 55%.

[0041] (3) After mixing evenly, stir, protect from light, and heat at 60°C for 24 h under nitrogen protection. After the reaction, the above solution was put into a dialysis bag, and dialyzed in deionized water for 1 day. Transfer it into a beaker, seal it with filter paper, and freeze-dry to obtain a dopamine and hyaluronic acid-modified fluorinated graphene sample (FGO-PDA-HA).

Embodiment 2

[0043](1) Prepare Tris-HCl buffered saline solution (TBS solution) with pH = 8.0: Accurately weigh the Tris-HCl solid, add ultrapure water to dissolve it, and adjust the pH to 8.0 with concentrated HCl.

[0044] (2) Accurately weigh 2 g of HA, dissolve 100 mg of FGO in 200 mL of TBS solution with pH=8.0, and sonicate for 40 minutes; add 500 mg of DA, and adjust the pH value to 8.0 with HCl; the oxygen content of FGO is 95%, The fluorine content is 5%.

[0045] (3) After mixing evenly, stir, protect from light, and heat at 80°C for 7 h under nitrogen protection. After the reaction, the above solution was put into a dialysis bag, and dialyzed in deionized water for 1 day. Transfer it into a beaker, seal it with filter paper, and freeze-dry to obtain a dopamine and hyaluronic acid-modified fluorinated graphene sample (FGO-PDA-HA).

Embodiment 3

[0047] (1) Prepare Tris-HCl buffered saline solution (TBS solution) with pH=10: Accurately weigh the Tris-HCl solid, add ultrapure water to dissolve it, and adjust the pH to 10 with concentrated HCl.

[0048] (2) Accurately weigh 1 g of HA, dissolve 100 mg of FGO in 200 mL of TBS solution with pH=10, and sonicate for 40 min; add 10 mg of DA-HCl, and use HCl to adjust the pH to 9.5; the oxygen content of FGO is 5%, the fluorine content is 95%.

[0049] (3) After mixing evenly, stir, protect from light, and heat at 25°C for 36 h under the protection of helium. After the reaction, the above solution was put into a dialysis bag, and dialyzed in deionized water for 1 day. Transfer it into a beaker, seal it with filter paper, and freeze-dry to obtain a dopamine and hyaluronic acid-modified fluorinated graphene sample (FGO-PDA-HA).

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Abstract

The invention discloses a drug-loaded synovial fluid additive. According to the invention, fluorinated graphene, dopamine and hyaluronic acid / sodium hyaluronate are used as main raw materials, and a fluorinated graphene nano-drug carrier coupled by polydopamine and modified by hyaluronic acid / sodium hyaluronate is synthesized through a one-step method; and after the fluorinated graphene nano-drugcarrier adsorbs a drug for treating arthritis, the fluorinated graphene nano-drug carrier is used as a compound additive to improve the lubricating property of a synovial fluid. The technical preparation method is simple; the composite additive has good biocompatibility, and can remarkably reduce the degradation speed of hyaluronic acid; the loaded medicine can effectively treat arthritis; and theantifriction and antiwear properties of the synovial fluid are greatly improved, so the service life of synovial fluid is prolonged.

Description

technical field [0001] The invention belongs to the technical fields of mechanical engineering and bioengineering, and in particular relates to a drug-loaded joint synovial fluid additive and its preparation method and application. Background technique [0002] Arthritis is a common chronic disease, known as "the number one disabling disease". There are more than 100 million arthritis patients in my country, and about 50% of the elderly over the age of 50 suffer from arthritis (see Sci. Rep., 2016, 6, 24393). Articular cartilage wear and inflammation caused by joint synovial fluid lubrication failure is an important cause of arthritis (see Adv. Funct. Mater., 2018, 1807559; Adv. Health. Mater., 2018, 1701463). When arthritic lesions occur, the content and molecular weight of hyaluronic acid in synovial fluid will decrease, and patients need to inject HA to achieve cartilage repair and viscosity compensation. However, the injected hyaluronic acid has insufficient compressiv...

Claims

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Application Information

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IPC IPC(8): A61K47/52A61K47/04A61K31/728A61K31/196A61P19/02A61P29/00
CPCA61K47/52A61K47/02A61K31/728A61K31/196A61P19/02A61P29/00A61K2300/00
Inventor 公培伟彭景一刘哲赵凯翟明珠李慧许文宇
Owner QUFU NORMAL UNIV
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