Device and method for detecting a specific analyte in a liquid sample and uses of said device
A technology for liquid samples and analytes, applied in biochemical equipment and methods, measurement devices, chemical instruments and methods, etc., can solve the problems of low detection variation, low detection sensitivity, high instrument complexity, etc., to achieve low detection variation, The effect of high detection accuracy and high detection specificity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0067] Example 1 - Rationale for the method for detecting a specific analyte in a liquid sample
[0068] The maximum reaction rate v of an enzymatic reaction max Depends on the temperature and pH of the solution in which the reaction is carried out. For a specific constant pH, v max Depends on the ambient temperature of the device according to the invention. If the ambient temperature is also constant (e.g., a constant room temperature of 25°C), then v max Considered to be a fully determined value. In this case, the measured reaction rate v of the substrate (analyte) depends on the concentration of the substrate (analyte) in solution (Michaelis-Menten theory). Applicable here is the following equation
[0069] v=(v max ·[Analyte]) / (k m +[Analyte])
[0070] where [analyte] is the concentration of the analyte, k m Indicates the Michaelis constant of the enzyme.
[0071] Therefore, a known v under certain conditions max and the known Michaelis constant k m In the case...
Embodiment 2
[0076] Embodiment 2-method for detecting urea in a liquid sample
[0077] For the detection of the analyte urea, the device according to the invention contains urease in the enzyme zone. like figure 1 As shown, urease catalyzes the conversion of urea to produce two gases, ammonia and carbon dioxide.
[0078] The acidification zone on the device according to the invention ensures that, firstly, the carbon dioxide produced does not go into solution as H 3 o + and HCO 3 - , but escape quantitatively. As a result, the detection sensitivity of the device for urea is improved.
[0079] In addition, acidification causes the ammonia produced to preferentially go into solution as NH 4 + and OH - . However, since ammonia has anyway a high tendency to dissolve in aqueous media even at neutral pH, acidification hardly shifts the equilibrium in the direction of dissolved ammonia. In other words, acidification hardly reduces ammonia production, which means that acidification has ...
Embodiment 3
[0085] Example 3 - Method for Detecting Lactate in Liquid Samples
[0086] Lactate oxidase selectively converts lactate to pyruvate and hydrogen peroxide (see figure 1 ). In the second step, hydrogen peroxide, due to its strong reducing effect, reacts with a solution of potassium permanganate slightly acidified with sulfuric acid. Redox reactions lead firstly to the decolorization of potassium permanganate solutions with an intense purple color and secondly to the formation of oxygen (see figure 1 ).
[0087] In order to react hydrogen peroxide with a solution of potassium permanganate acidified with sulfuric acid, the device therefore also requires an oxidation zone containing at least one oxidizing agent (e.g. due to dissolved KMnO 4 and the resulting MnO 4 - ) and at least one acid (H 3 o + , for example, due to dissolved H 2 SO 4 resulting in H 3 o + ).
[0088] Therefore, the product (quantity) of the number and volume of oxygen gas bubbles depends on the conc...
PUM
| Property | Measurement | Unit |
|---|---|---|
| length | aaaaa | aaaaa |
| height | aaaaa | aaaaa |
| diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More