Bovine vesicular stomatitis detection kit and preparation method thereof

Abstract

The invention provides a bovine vesicular stomatitis detection kit and a preparation method thereof, and belongs to the technical field of molecular biology. The detection kit comprises a solid phase,a VSV antigen is fixed to the solid phase, the VSV antigen comprises recombinant P1-87 protein, the P1-87 protein comprises an SEQ ID No.1 amino acid sequence, a stop solution and a liquid phase containing a detection antibody, and the detection antibody comprises an HRP-anti-bovine IgG antibody used for detecting IgG. The kit provided by the invention establishes an indirect ELISA diagnosis method by bovine vesicular stomatitis virus truncated P protein, an antigen determinant in the recombinant protein can be exposed, the antigen titer is improved, the antigenic specificity is improved, andthe detection rate is improved.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a detection kit for bovine vesicular stomatitis and a preparation method thereof. Background technique [0002] Vesicular stomatitis (VS) is a highly contagious zoonotic disease caused by vesicular stomatitis virus (VSV), which mainly causes vesicular disease in infected animals clinically. It is characterized by the appearance of vesicular stomatitis lesions on the mucous membrane or skin of the oral cavity, lips, tongue, teats, and hooves of infected animals. VSV is mainly transmitted through the saliva and blister fluid of sick animals. In addition, the disease can also cause meningoencephalitis in infected animals, with obvious neurological symptoms. [0003] The diagnostic method of VSV has gone through a process of gradual development. It was first diagnosed through the observation of clinical symptoms. Since the disease is similar to clinical sympto...

AI Technical Summary

Problems solved by technology

[0003] The diagnostic method of VSV has gone through a process of gradual development. It was first diagnosed through the observation of clinical symptoms. Since the disease is similar to clinical symptoms such as foot-and-mouth disease and swine vesicular disease, the reliability of the diagnosis obtained from clinical symptom observation is not high, and it is easy to appear. Case of misdiagnosis

Subsequently, immunology and molecular biology techniques were gradually developed, which was an era-changing reform for large-scale veterinary clinical testing. These testing methods include neutralization test, agar diffusion test, enzyme-linked immunosorbent assay (ELISA), PCR detection methods, etc., these detection methods have good detection results, but they have high requirements for experimental conditions, cannot be tested in general farms, and the detection cost is relatively high, requiring professional laboratory personnel to operate. In view of these Insufficient and limited, it is very necessary to establish a simple and easy-to-use detection method for clinical diagnosis, epidemic monitoring and epidemiological investigation of the disease

Images