Healing and growth-promoting agent for grafting of gleditsia sinensis and preparation method and application thereof
A growth agent and acacia technology, which is applied in the field of acacia grafting healing growth promoters and preparations, can solve the problems that the healing agent does not completely meet the needs of acacia grafting, improve the survival rate of grafting, and complicated and complicated operations, so as to improve the survival rate of grafting and improve the quality of grafting , the effect of less dosage
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Embodiment 1
[0040] Embodiment 1: a kind of acacia grafting healing growth-promoting agent, is prepared from the raw material of following concentration ratio: agent A growth hormone, agent B fungicide, agent C nutrient, agent D film-forming agent; Wherein: the solvent of agent A It is 1% dilute hydrochloric acid and distilled water or purified water, based on the total volume of agent A, agent A is: 40-60mg / L of 6-benzylaminopurine 6-BA, 40-60mg / L of kinetin KT and 30 -120 mg / L Potassium Indolebutyrate IBA-K;
[0041] Among them, 6-benzylaminopurine is a safe and efficient plant growth regulator, which has the functions of accelerating cell division, accelerating cell proliferation, inducing callus, promoting plant growth and thickening, and improving plant disease resistance.
[0042] Indolebutyric acid (potassium), a root-promoting plant growth regulator, can promote cell differentiation and division, promote the differentiation of vascular system, accelerate the growth of main roots, a...
Embodiment 2
[0050] Embodiment 2: choose the Chinese honey locust fine variety---" Shuai Ding " to do the scion and carry out grafting, the scion bud is plump, without damage by disease and insect, without mechanical damage, stock is the same provenance 3-year-old Chinese honey locust sowing seedling, stock average ground diameter is 2.2cm, at 20cm for grafting. At first carry out the single factor test of plant growth hormone, to determine the kind and concentration of regulator suitable for saponins grafting, the using method of healing accelerant is the same as example 1. The experimental conditions and results are shown in Table 1-Table 4.
[0051] The test results showed that compared with the control, at a certain concentration, 6-BA, KT, GA 3 , IBA-K can promote healing, increase the survival rate of grafting, and promote the growth of grafted seedlings. Among them, the suitable concentration of 6-BA is 40-60mg / L, the suitable concentration of KT is 40-60mg / L, the suitable concent...
Embodiment 3
[0060] Embodiment 3: choose acacia fine variety---" Shuai Ding " to do scion and carry out grafting, and scion bud is plump, without damage by disease and insect, without mechanical damage, and rootstock is the same provenance 3-year-old acacia seedling, and rootstock average ground diameter is 2.2cm, at 20cm for grafting. The combination of 6-BA, KT and IBA-K was selected for orthogonal experiment, and the appropriate combination concentration was obtained through orthogonal experiment. The using method of healing accelerator is the same as embodiment 1. The orthogonal design is shown in Table 5, and the results are shown in Table 6.
[0061] It can be seen from table 6 that among the 9 treatments, the 6 mixed solution was treated, the concentration of 6-BA was 50mg / L, the concentration of KT was 40mg / L, the suitable concentration of IBA-K was 120mg / L, and the grafting survival rate of saponins was the highest, which was 95.56%. The promotion effect on grafted seedlings was...
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