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A method for yeast fermentation using fresh Codonopsis pilosula as raw material

A technology of yeast and Codonopsis pilosula, applied in the direction of microorganism-based methods, plant raw materials, biochemical equipment and methods, etc., can solve the problems of no fresh Codonopsis pilosula, etc., and achieve the improvement of the content of some index components, the increase of clearance rate, and the resistance The effect of enhanced oxidation activity

Active Publication Date: 2022-02-15
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no literature record and application of fresh Codonopsis pilosula fermented by yeast

Method used

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  • A method for yeast fermentation using fresh Codonopsis pilosula as raw material
  • A method for yeast fermentation using fresh Codonopsis pilosula as raw material
  • A method for yeast fermentation using fresh Codonopsis pilosula as raw material

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Comparative Study on Chemical Components of Fresh Codonopsis Codonopsis and Codonopsis Codonopsis

[0030] 15 samples of fresh Codonopsis pilosula were processed according to the primary processing method of traditional Codonopsis pilosula origin, and 15 samples of Codonopsis pilosula from different origins were obtained. Table 1 shows sample information.

[0031] Table 1

[0032] Sample serial number collection place collection time S1 Fengjia Village, Xinzhai Town, Weiyuan County, Gansu Province 2014-10-5 S2 Sample 1 from Miaozui Village, Xinzhai Town, Weiyuan County, Gansu Province 2014-10-5 S3 Sample 2 from Miaozui Village, Xinzhai Town, Weiyuan County, Gansu Province 2014-10-5 S4 Gaobaozi Village, Gaoling Township, Longxi County, Gansu Province 2014-10-6 S5 Lijiaying, Shouyang Town, Longxi County, Gansu Province 2014-10-6 S6 Chenjiazui Village, Maquan Township, Zhang County, Gansu Province 2014-10-7 S7 S...

Embodiment 2

[0039] Comparison of Chemical Components and Activities of Fermented Fresh Codonopsis Codonopsis and Fresh Codonopsis Codonopsis

[0040] 2.1 Determination of the fermentation end point

[0041] The end point of the fermentation is determined by the changes in the number of bacteria, changes in pH value, water extracts, and alcohol extracts during the fermentation process. Table 3 shows the change of pH value of fresh Codonopsis pilosula with different bacteria additions during different fermentation time. Table 4 shows the changes in the number of fresh Codonopsis pilosula with different amounts of bacteria added during different fermentation times. Table 5 shows the changes in water extract content (%) of fresh Codonopsis pilosula with different bacteria additions during different fermentation times. Table 6 shows the changes in alcohol extract content (%) of fresh Codonopsis pilosula with different bacteria additions during different fermentation times.

[0042] table 3 ...

Embodiment 3

[0062] A method for yeast fermentation using fresh Codonopsis pilosula as raw material:

[0063] 1) The selected fermentation raw material is fresh Codonopsis pilosula, that is, fresh and plump Codonopsis pilosula newly excavated.

[0064] 2) Select fresh white Codonopsis ginseng without rot and deterioration. There are a lot of soil and harmful microorganisms attached to the fibrous roots on the surface of the raw materials. The crushed fresh Codonopsis pilosula is subpackaged and stored at -20 degrees to obtain fresh Codonopsis pilosula homogenate.

[0065] 3) Put a small amount of Angel dry active yeast in a sterile petri dish, dissolve it with a small amount of sterile water, burn the inoculation loop on an alcohol lamp until it is red hot, and pick a small amount of yeast aseptically after cooling Bacterial samples were inoculated on YEPD solid medium (Yeast Extract Peptone Dextrose Medium): peptone 2% yeast extract 1% glucose 1% agar 2%, 94% distilled water with natural...

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Abstract

The invention discloses a yeast fermentation method using fresh Codonopsis pilosula as a raw material. The fresh Codonopsis pilosula is broken and homogenized, sterilized and then fermented with activated yeast, the fermented product is sterilized, taken out, dried and pulverized to obtain Fermented fresh Codonopsis pilosula product. The beneficial effects of the present invention are: the fermented fresh Codonopsis pilosula product prepared by the method provided by the present invention, compared with unfermented fresh Codonopsis pilosula and Codonopsis pilosula medicinal materials, the content of total polysaccharides, total flavonoids, and total triterpenes increases, DPPH free radicals, superoxide The scavenging rate of anion and hydroxyl radicals increased. From the comparison results, it can be seen that the fermentation process parameters are controllable throughout the whole process, the content of some index components is significantly higher than that of fresh Codonopsis pilosula, and the antioxidant activity is significantly enhanced. Fermenting fresh Codonopsis pilosula can make full use of the water and sugar in fresh Codonopsis pilosula to form a natural culture medium for yeast, and the fermentation process is simplified and controllable. Fermentation of fresh Codonopsis pilosula can change the content of components, produce microbial metabolites, and further change the activity.

Description

technical field [0001] The invention relates to the technical field of processing traditional Chinese medicinal materials, in particular to a method for yeast fermentation using fresh Codonopsis pilosula as raw material. Background technique [0002] Although the use of fresh medicine is rare now, it was widely used in ancient times, especially popular among folk doctors. It can be collected and used at will in the mountains and on the roadside, which is a good medicine for curing diseases. Ancient herbal books and famous doctors of all dynasties have described and praised fresh medicine. In the earliest herbal book "Shen Nong's Materia Medica", there is a saying that "the living is the best" for fresh medicine. It is frequently mentioned in various herbal books, "dry is easy, fresh is better". "Fresh ones are more powerful", "Fresh ones are smashed into juice, which is especially good for medicine. Dried ones can also be used, but they are not as powerful as fresh ones."...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/344C12N1/18C12R1/865A61K125/00
CPCA61K36/344C12N1/18A61K2236/19A61K2236/13A61K2236/31
Inventor 胡芳弟贾旭森苟于强白瑞斌王燕萍
Owner LANZHOU UNIVERSITY